CAJ | 학술논문

为探讨空肠弯曲菌生物膜耐药机理，采用硫酸-蒽酮法测定了浮游菌和生物膜菌胞外多糖的产量。结果表明，两种状态细菌合成的水溶性胞外多糖量，随着时间的延长，呈现出先增加后下降的趋势。在同一时间点，生物膜菌黏附状态均显著低于浮游状态（P ＜0．05）；水不溶性胞外多糖除了黏附状态合成量高于浮游状态外，不具有明显的规律。不同状态细菌合成胞外多糖差异显著，生物膜所处的酸性环境、营养物质的耗尽等可能是产生差异的原因。
위탐토공장만곡균생물막내약궤리，채용류산-은동법측정료부유균화생물막균포외다당적산량。결과표명，량충상태세균합성적수용성포외다당량，수착시간적연장，정현출선증가후하강적추세。재동일시간점，생물막균점부상태균현저저우부유상태（P ＜0．05）；수불용성포외다당제료점부상태합성량고우부유상태외，불구유명현적규률。불동상태세균합성포외다당차이현저，생물막소처적산성배경、영양물질적모진등가능시산생차이적원인。
To investigate the mechanism of biofilm-resistant Campylobacter j ejuni ,sulfuric acid-anthrone method for the determination of planktonic bacteria and biofilm bacteria extracellular polysaccharide yields. The experiment showed that quantity of water soluble extracellular polysaccharide improved then decreased with the time lasted.At the same time,adhesion bacteria is significantly lower than planktonic bacteria,(P<0.05),but the amouts of water insoluble extracellular polysaccharide were absolutely different.There was a significant differences of the ability in syntheses of exopolysaccharide between different conditions, reasons causing the differences may be acidic conditions where the biofilm grows and nutrients are deple-ted.