CAJ | 학술논문

为评估奶山羊乳房炎灭活疫苗的免疫效果，以大肠埃希菌、金黄色葡萄球菌、凝固酶阴性表皮葡萄球菌及产色葡萄球菌为材料，经甲醛37℃灭活，按照6∶1（V／V）与铝胶盐充分混匀，分别经腹股沟皮下、颈部皮下注射途径，免疫泌乳期奶山羊，免疫剂量均为2 mL，共免疫2次，每次间隔14 d。分别在免疫前及第1次免疫后10、24、60、180 d 采集血样和乳样，ELISA 测定抗体效价，用快速诊断液检测隐性乳房炎，统计患病奶山羊的数量。结果显示，与对照组相比，两次免疫后血液及乳汁中针对大肠埃希菌、金黄色葡萄球菌、凝固酶阴性表皮葡萄球菌及产色葡萄球菌的抗体效价含量均显著增加（P ＜0．05），抗体维持时间长；颈部皮下免疫组免疫后180 d，腹股沟皮下免疫组免疫后的60 d，隐性乳房炎奶山羊数量均明显下降。结果表明，本研究制备的奶山羊乳房炎疫苗不仅能够刺激机体产生较好的体液免疫应答，而且还对隐性乳房炎有显著的治疗效果。
위평고내산양유방염멸활역묘적면역효과，이대장애희균、금황색포도구균、응고매음성표피포도구균급산색포도구균위재료，경갑철37℃멸활，안조6∶1（V／V）여려효염충분혼균，분별경복고구피하、경부피하주사도경，면역비유기내산양，면역제량균위2 mL，공면역2차，매차간격14 d。분별재면역전급제1차면역후10、24、60、180 d 채집혈양화유양，ELISA 측정항체효개，용쾌속진단액검측은성유방염，통계환병내산양적수량。결과현시，여대조조상비，량차면역후혈액급유즙중침대대장애희균、금황색포도구균、응고매음성표피포도구균급산색포도구균적항체효개함량균현저증가（P ＜0．05），항체유지시간장；경부피하면역조면역후180 d，복고구피하면역조면역후적60 d，은성유방염내산양수량균명현하강。결과표명，본연구제비적내산양유방염역묘불부능구자격궤체산생교호적체액면역응답，이차환대은성유방염유현저적치료효과。
In order to evaluate the immune effect of the inactivated vaccine against mastitis in dairy goats, E.coli,Staphylococcus aureus ,coagulase negative Staphylococcus epidermidis and Staphylococcus chro-mogenes were inactivated by using methanal at 37℃ and then thoroughly mixed with adjuvant aluminum hydroxide with a ratio of 6:1 (V/V).Each lactating dairy goat was inoculated subcutaneously via neck or inguen twice with an interval of 14 d and the dosage was 2 ml each time.Blood and milk samples were col-lected before and post inoculation on day 10,24,60 and 180.The levels of antibodies were measured by an indirect ELISA while the numbers of goats suffering subclinical mastitis were measured by a rapid diagnos-tic reagent.The results showed that antibodies against E.coli,Staphylococcus aureus ,coagulase negative Staphylococcus epidermidis and Staphylococcus chromogenes in blood and milk were significantly increased compared with control after the twice inoculations (P < 0.05),and the antibodies could last long.The numbers of goats suffering subclinical mastitis of the neck and inguen inoculated groups were always less than control until the day 180 and 60 post inoculation respectively.In conclusion,the inactivated vaccine a-gainst mastitis developed in this study could not only stimulate the body to produce high-level antibodies but also has a significant therapeutic effect to subclinical mastitis.