CAJ | 학술논문

为研究牛乳金黄色葡萄球菌 SP-3分离株携带超抗原肠毒素的基因型，设计合成了金黄色葡萄球菌超抗原肠毒素基因 SEA-SEE 及 TSST-1特异性引物，并以其固有基因 femA 和 femB 为阳性对照，以分离菌株 DNA 为模板，经 PCR 扩增菌株携带的毒素基因。结果表明，SP-3分离株和阳性对照菌株均含有femA 和 femB 基因，且牛乳分离株携带 sea、sec、sed 和 tst-14种超抗原肠毒素基因，为开展动物源超抗原毒素基因系统分型及有效防控金黄色葡萄球菌奶牛乳腺炎奠定基础。
위연구우유금황색포도구균 SP-3분리주휴대초항원장독소적기인형，설계합성료금황색포도구균초항원장독소기인 SEA-SEE 급 TSST-1특이성인물，병이기고유기인 femA 화 femB 위양성대조，이분리균주 DNA 위모판，경 PCR 확증균주휴대적독소기인。결과표명，SP-3분리주화양성대조균주균함유femA 화 femB 기인，차우유분리주휴대 sea、sec、sed 화 tst-14충초항원장독소기인，위개전동물원초항원독소기인계통분형급유효방공금황색포도구균내우유선염전정기출。
To survey the enterotoxin genotypes of Staphylococcus aureus SP-3 strain isolated from the raw milk of subclinical mastitis cow,we developed specific PCR primers for five classical enterotoxin types SEA through SEE and toxin shock syndrome toxin 1 (TSST-1)type genes,as positive control with the femA and femB gene of S.aureus .The type strain (ATCC126000)and 834 strain were used as reference strains.Genomic DNA of the enterotoxigenic S.aureus were used as the template,five classical enterotox-ins and TSST-1 genes produced by S.aureus were detected by PCR amplification.The result indicated that the SP-3 isolate and the reference strains harbored femA and femB,and the SP-3 isolate from milk harbored sea,sec,sed and tst-1 superantigenic toxin genes.The study would lay a foundation for further studies on the superantigenic toxin genotyping in animal source S.aureus isolates and for preventing and controlling the infection of S.aureus in mastitis cows.