CAJ | 학술논문

通过对碧桃花瓣组织进行转录组测序，获得1556684条序列，平均读长446 bp，共计695．34 Mb数据，其中1492289条高质量序列经拼接后得到22762个重叠群。以拼接后的重叠群作为参考序列，利用SNP／InDel分析软件检测碧桃花瓣组织转录组中的核苷酸变异位点，共得到9836个SNP和1550个InDel位点。在SNP位点中，转换占62．55％、颠换占37．45％，A／G的变异最为丰富，C／G的变异最少。在InDel位点中，有34．71％的位点为单核苷酸插入、缺失突变。
통과대벽도화판조직진행전록조측서，획득1556684조서렬，평균독장446 bp，공계695．34 Mb수거，기중1492289조고질량서렬경병접후득도22762개중첩군。이병접후적중첩군작위삼고서렬，이용SNP／InDel분석연건검측벽도화판조직전록조중적핵감산변이위점，공득도9836개SNP화1550개InDel위점。재SNP위점중，전환점62．55％、전환점37．45％，A／G적변이최위봉부，C／G적변이최소。재InDel위점중，유34．71％적위점위단핵감산삽입、결실돌변。
We identified single-nucleotide polymorphisms (SNP) and insertion deletion length polymorphism (InDel)in the transcriptome from ornamental peach flower petals using the sequences generated by the Roche 454 GS-FLX sequencer .A total of 1 556 684reads were generated with an average length of 446 bp.After filtering poor quality reads , 1 492 289 high quality reads were obtained and were further assembled into 22 762 contigs .Using these contigs as a reference sequences , 9 836 SNPs and 1 550 InDels were identified.In the SNPs,transitions and transversions were accounted for 62.55%and 37 .45%, respectively.In these SNPs, A/T transitions were the most abundant , and C/G transitions were much rarer than any of the other SNP types. In the InDels, mononucleotide changes amounted for 34.71%.