CAJ | 학술논문

目的：检测不同浓度重组人白介素－1β（recombinant human interleukin－1β，rhIL－1β）对体外培养人牙周膜细胞（human periodontal ligament cells，HPDLCs）表达骨保护因子（osteoprotegerin，OPG）的影响，研究rhIL－1β水平对牙周膜细胞骨向分化的作用。方法：正畸需要而拨除的健康前磨牙牙周膜，体外传代培养HPDLCs；ELISA法和RT－PCR法测定不同浓度rhIL－1β（0、5、10、15μg／L）下HPDLCs的OPG分泌量及mRNA表达。结果：ELISA和RT－PCR法检测结果显示，5、10、15μg／L rhIL－1β作用于HPDLCs均会下调其OPG蛋白分泌和mRNA表达。同一时间点与0μg／L对照组比较，5、10、15μg／L组OPG蛋白分泌和mRNA表达依此下调，差异有统计学意义（P＜0．05）。结论：rhIL－1β水平升高会增强对HPDLCs的OPG表达抑制，对牙槽骨健康产生不利影响。
목적：검측불동농도중조인백개소－1β（recombinant human interleukin－1β，rhIL－1β）대체외배양인아주막세포（human periodontal ligament cells，HPDLCs）표체골보호인자（osteoprotegerin，OPG）적영향，연구rhIL－1β수평대아주막세포골향분화적작용。방법：정기수요이발제적건강전마아아주막，체외전대배양HPDLCs；ELISA법화RT－PCR법측정불동농도rhIL－1β（0、5、10、15μg／L）하HPDLCs적OPG분비량급mRNA표체。결과：ELISA화RT－PCR법검측결과현시，5、10、15μg／L rhIL－1β작용우HPDLCs균회하조기OPG단백분비화mRNA표체。동일시간점여0μg／L대조조비교，5、10、15μg／L조OPG단백분비화mRNA표체의차하조，차이유통계학의의（P＜0．05）。결론：rhIL－1β수평승고회증강대HPDLCs적OPG표체억제，대아조골건강산생불리영향。
Objective:To determine the effects of rhIL-1βon osteoprotegerin expression of cultured human periodon-tal ligament cells (HPDLCs) in vitro,and reach the role of rhIL-1βon the HPDLCs during their osteogenic differentiation. Method:HPDLCs were derived from periodontal membrane of healthy premolars that were extracted for orthodontics and cultured in vitro and secretion levels of the OPG mRNA expression were determined by ELISA and RT-PCR methods under the effect of rhIL-1βat different concentrations (0、5、10、15μg/L). Result:ELISA and RT-PCR assay showed that rhIL-1βact on HPDLCs reduced the OPG protein secretion and mRNA expression in the 5、10、15 μg/L groups,with negative positively correlated with concentrations. As compared with 0 μg/L control group at the same time,the difference was sig-nificant(P<0.05) in the 5、10、15μg/L groups. Conclusion:rhIL-1βcan inhibition the OPG expression in HPDLCs at ex-perimental concentration and play an adverse role in the alveolar bone reconstruction.