CAJ | 학술논문

以葡萄糖为标准品，利用大孔吸附树脂分离纯化玉竹多糖，结果表明AB-8大孔吸附树脂分离纯化玉竹多糖的最佳上样浓度为1.0 mg/mL，最佳洗脱浓度为75%乙醇，最佳流速为1.0 mL/min，洗脱液体积为上样的10 BV，玉竹粗多糖的纯度从65.23%提高到78.64%；D-101大孔吸附树脂分离纯化玉竹多糖的最佳上样浓度为0.6 mg/mL，最佳洗脱浓度为50%乙醇，最佳流速为1.0 mL/min，洗脱液体积为上样的10.5 BV，玉竹粗多糖的纯度从65.23%提高到73.79%。AB-8大孔吸附树脂对玉竹多糖的分离纯化效果优于D-101大孔吸附树脂。
이포도당위표준품，이용대공흡부수지분리순화옥죽다당，결과표명AB-8대공흡부수지분리순화옥죽다당적최가상양농도위1.0 mg/mL，최가세탈농도위75%을순，최가류속위1.0 mL/min，세탈액체적위상양적10 BV，옥죽조다당적순도종65.23%제고도78.64%；D-101대공흡부수지분리순화옥죽다당적최가상양농도위0.6 mg/mL，최가세탈농도위50%을순，최가류속위1.0 mL/min，세탈액체적위상양적10.5 BV，옥죽조다당적순도종65.23%제고도73.79%。AB-8대공흡부수지대옥죽다당적분리순화효과우우D-101대공흡부수지。
Glucose is used as standerd in the experiment. AB-8 macroporous adsorption resin to extract and purify polysaccharide of polygonatum odoratum. The best condition of extracting and purifying polygonatum odoratum, the best concentration for samples was 1.0 mg/mL, the best elution concentration was 75 percent of ethanol, the best velocity was 1.0 mL/min, and the elution liquid volume was 10 BV. The purification degree of crude polysaccharid in polygonatum odoratum increased from 65.23 % to 78.64 %. However, using D-101 macroporous adsorption resin to extract and purify poly saccharide of polygonatum odoratum. The best condition of extracting and purifying polygonatum odoratum, the best concentration for samples was 0.6 mg/mL, the best elution concentration was 50 percent of ethanol, the best velocity was 1.0 mL/min, and the elution liquid volume was 10.5 BV. The purification degree of crude polysaccharid in polygonatum odoratum increased from 65.23%to 73.79%. The result showed that the effect of extracting and purifying polysaccharide of polygonatum odoratum with AB-8macroporous adsorption resin was better than D-101 macroporous adsorption resin.