CAJ | 학술논문

结核分支杆菌（MTB）是引起多种宿主感染结核病的重要病原菌，由于结核分支杆菌耐药菌株的产生，导致结核病在世界范围内呈高发趋势。由于 MTB 检出率较低，开发一种简单快速的诊断方法迫在眉睫。指数富集配体系统进化技术（SELEX）的原理是从随机寡核苷酸文库中筛选到能特异性结合靶物质的寡核苷酸配体（称为适配体）。论文对近几年国内外通过 SELEX 技术对结核分支杆菌筛选出的适配体做一综述，适配体的靶物质分别为分泌蛋白 MPT64，CFP10-ESAT6蛋白，多磷酸激酶2（PPK2）以及结核分支杆菌（H37Rv 株）整个菌体。体外筛选的结核分支杆菌适配体，能对结核病做出早期诊断，并能作为 MTB疫苗的免疫佐剂，在临床诊断及药物研究等方面有很大应用前景。
결핵분지간균（MTB）시인기다충숙주감염결핵병적중요병원균，유우결핵분지간균내약균주적산생，도치결핵병재세계범위내정고발추세。유우 MTB 검출솔교저，개발일충간단쾌속적진단방법박재미첩。지수부집배체계통진화기술（SELEX）적원리시종수궤과핵감산문고중사선도능특이성결합파물질적과핵감산배체（칭위괄배체）。논문대근궤년국내외통과 SELEX 기술대결핵분지간균사선출적괄배체주일종술，괄배체적파물질분별위분비단백 MPT64，CFP10-ESAT6단백，다린산격매2（PPK2）이급결핵분지간균（H37Rv 주）정개균체。체외사선적결핵분지간균괄배체，능대결핵병주출조기진단，병능작위 MTB역묘적면역좌제，재림상진단급약물연구등방면유흔대응용전경。
Mycobacterium tuberculosis (MTB)is the important pathogen of tuberculosis which can infect a variety of hosts,because of the generation of Mycobacterium tuberculosis drug-resistant strains,tuberculo-sis spreads worldwide,shows a high incidence trends.Due to the low detection rate of MTB,developing a simple and rapid diagnostic method is urgent.The principle of systematic evolution of ligands by exponen-tial enrichment (SELEX)is selecting the oligonucleotide ligands (called aptamers)specifically binding to the target substance from the random oligonucleotides library.In this paper,we summarized the national and abroad researchs on aptamers in Mycobacterium tuberculosis recently,the targets of these aptamers are MPT64,CFP10-ESAT6,PPK2 and the whole H37Rv strain.These aptamers can make an early diagnosis of TB and as an immunologic adjuvant of MTB vaccine,so they will have great potential applications in ar-eas of clinical diagnosis and drug research.