CAJ | 학술논문

通过观察不同方剂对小鼠感染弓形虫后脾 IL-2含量的影响，确定对弓形虫病治疗效果最佳的复方中药。将昆明系小鼠随机分为中药复方方剂组（A、B、C 组）、复方 SMZ 组（D 组）、模型组（E 组）和对照组（F 组）。用 RH 株弓形虫速殖子腹腔感染小鼠，2 h 后灌胃给药，其中 A、B、C 3个组每只每次0．5 mL 自拟弓形虫汤剂；D 组每只每次0．5 mL 复方 SMZ 混悬液300 mg／（kg· d－1），疗程15 d。采用双抗夹心ELISA 法，观察不同方剂对小鼠脾细胞 IL-2含量的影响，并对照不同方剂之间的差异，采用 F 检验和 q 检验进行统计分析。结果表明，A、B、C 组和 D 组 IL-2活性水平均随着时间延长而逐渐升高；A、C、D 组与 F组有显著性差异；B 组 IL-2水平活性保持了较高值，与 E 组差异极显著。说明3种自拟中药复方方剂均能显著提高弓形虫感染小鼠脾细胞 IL-2的含量，增强小鼠免疫功能，其中复方二提高最显著，对 IL-2活性影响最大。
통과관찰불동방제대소서감염궁형충후비 IL-2함량적영향，학정대궁형충병치료효과최가적복방중약。장곤명계소서수궤분위중약복방방제조（A、B、C 조）、복방 SMZ 조（D 조）、모형조（E 조）화대조조（F 조）。용 RH 주궁형충속식자복강감염소서，2 h 후관위급약，기중 A、B、C 3개조매지매차0．5 mL 자의궁형충탕제；D 조매지매차0．5 mL 복방 SMZ 혼현액300 mg／（kg· d－1），료정15 d。채용쌍항협심ELISA 법，관찰불동방제대소서비세포 IL-2함량적영향，병대조불동방제지간적차이，채용 F 검험화 q 검험진행통계분석。결과표명，A、B、C 조화 D 조 IL-2활성수평균수착시간연장이축점승고；A、C、D 조여 F조유현저성차이；B 조 IL-2수평활성보지료교고치，여 E 조차이겁현저。설명3충자의중약복방방제균능현저제고궁형충감염소서비세포 IL-2적함량，증강소서면역공능，기중복방이제고최현저，대 IL-2활성영향최대。
To find the best Chinese medicine formuale with effect to cure toxoplasmosis,the effect of them on the spleen IL-2 content in mice infected with Toxoplasma gondii was observed.Kunming strain mice were randomly divided into Chinese medicine formuale groups (A,B,C group),compound SMZ group(D group),model group(E group)and control group(F group).The mice were infected with Toxoplasma gondii intraperitoneally with tachyzoites of the RH strain,and irrigating medicine into stomach after 2 h. Every mouse in A,B,C groups received 0.5 mL self-designed Chinese herb formula everyday,0.5 mL com-pound SMZ suspension liquid every day(300 mg/kg)in D groups,the period was 15 days.The effects of medicine on spleen IL-2 contents were detected by ELISA,and the differences among different prescrip-tions were analyzed by F-test and q test.The result showed that the level of IL-2 activity in group A,B,C and group D were increased with time.There were significant differences between groups A,C,D and F group,the content of IL-2 in B group kept higher level,and had a very significant difference with that of E group.Three Chinese medicine formuale could improve the contents of spleen IL-2 in mice infected with Toxoplasma gondii ,enhance the immune function in mice,the most significant enhancement was the sec-ond compound group,had the most influence on IL-2.