食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2014年
23期
17-20
,共4页
牛乳铁蛋白素%Jurkat细胞%细胞凋亡
牛乳鐵蛋白素%Jurkat細胞%細胞凋亡
우유철단백소%Jurkat세포%세포조망
Lfcin B%Jurkat cell%apoptosis
研究了LfcinB作为免疫强化剂以及营养强化剂对肿瘤细胞的抑制作用。主要应用CCK-8法检测LfcinB对人T淋巴细胞白血病细胞（Jurkat细胞）活力的影响，应用双标记流式细胞术分析LfcinB抑制Jurkat细胞生长的作用方式。研究结果证实，LfcinB对Jurkat细胞增殖的影响与LfcinB的作用时间和浓度呈正相关，而且LfcinB主要时通过诱导Jurkat细胞发生早期凋亡来抑制其增殖，并且其诱导Jurkat细胞早期凋亡的最佳浓度和时间分别为200μg/mL和48 h。
研究瞭LfcinB作為免疫彊化劑以及營養彊化劑對腫瘤細胞的抑製作用。主要應用CCK-8法檢測LfcinB對人T淋巴細胞白血病細胞（Jurkat細胞）活力的影響，應用雙標記流式細胞術分析LfcinB抑製Jurkat細胞生長的作用方式。研究結果證實，LfcinB對Jurkat細胞增殖的影響與LfcinB的作用時間和濃度呈正相關，而且LfcinB主要時通過誘導Jurkat細胞髮生早期凋亡來抑製其增殖，併且其誘導Jurkat細胞早期凋亡的最佳濃度和時間分彆為200μg/mL和48 h。
연구료LfcinB작위면역강화제이급영양강화제대종류세포적억제작용。주요응용CCK-8법검측LfcinB대인T림파세포백혈병세포（Jurkat세포）활력적영향，응용쌍표기류식세포술분석LfcinB억제Jurkat세포생장적작용방식。연구결과증실，LfcinB대Jurkat세포증식적영향여LfcinB적작용시간화농도정정상관，이차LfcinB주요시통과유도Jurkat세포발생조기조망래억제기증식，병차기유도Jurkat세포조기조망적최가농도화시간분별위200μg/mL화48 h。
Inhibition of cancer cells from LfcinB as immune-enhancer and nutrient supplements was researched. This experiment measured the vitality of Jurkat cells by CCK-8 and analysed the mode of action from LfcinB effect to human acute T lymphocyte leukemia cells (Jurkat cells) by double marker flow cytometry. According to the results, the effects of LfcinB to Jurkat cells on proliferation inhibition were positively related to the action concentration and time. And the proliferation inhibition of Jurkat cells by LfcinB was mainly induced by the early apoptosis ratio, and the best concentration and time to induce Jurkat cells early apoptosis was 200μg/mL and 48 h.
