CAJ | 학술논문

目的：观察丹参对大鼠心肌缺血再灌注损伤的保护作用。方法取健康雄性大鼠150只，根据数字表法随机分为五组：即假手术组、模型组、丹参高剂量组（100 mg／kg）、中剂量组（30 mg／kg）、低剂量组（10 mg／kg）。丹参组分别于术前灌胃给药，每天1次，连续3 d；假手术组和模型组灌以等量0．9％氯化钠注射液。采用在体大鼠结扎冠状动脉30 min然后松扎冠状动脉180 min造心肌缺血再灌注损伤模型。测定心肌梗死范围，测定血清磷酸肌酸激酶（ CK）、乳酸脱氢酶（ LDH）。结果模型组与假手术组相比，心肌梗死范围及血清CK、LDH活性均显著增加（t＝14．382、21．460，均P＜0．05）。不同剂量丹参组均能明显缩小大鼠心肌缺血再灌注损伤的心肌梗死范围，明显降低血清CK，与模型组比较差异均有统计学意义（ t＝7．426、6．891、11．274，均P＜0．05）。不同剂量丹参组均能明显降低血清LDH，与模型组比较差异均有统计学意义（ t＝22．436、10．843、16．252，均P＜0．05）。结论丹参对心肌缺血再灌注大鼠具有抗氧化应激的作用，对大鼠心肌缺血再灌注损伤具有保护作用。
목적：관찰단삼대대서심기결혈재관주손상적보호작용。방법취건강웅성대서150지，근거수자표법수궤분위오조：즉가수술조、모형조、단삼고제량조（100 mg／kg）、중제량조（30 mg／kg）、저제량조（10 mg／kg）。단삼조분별우술전관위급약，매천1차，련속3 d；가수술조화모형조관이등량0．9％록화납주사액。채용재체대서결찰관상동맥30 min연후송찰관상동맥180 min조심기결혈재관주손상모형。측정심기경사범위，측정혈청린산기산격매（ CK）、유산탈경매（ LDH）。결과모형조여가수술조상비，심기경사범위급혈청CK、LDH활성균현저증가（t＝14．382、21．460，균P＜0．05）。불동제량단삼조균능명현축소대서심기결혈재관주손상적심기경사범위，명현강저혈청CK，여모형조비교차이균유통계학의의（ t＝7．426、6．891、11．274，균P＜0．05）。불동제량단삼조균능명현강저혈청LDH，여모형조비교차이균유통계학의의（ t＝22．436、10．843、16．252，균P＜0．05）。결론단삼대심기결혈재관주대서구유항양화응격적작용，대대서심기결혈재관주손상구유보호작용。
Objective To observe the protective effect of Salvia miltiorrhiza on myocardial ischemia reperfu-sion injury in rats.Methods 150 healthy male rats were randomly divided into 5 groups:sham operation group,model group,Radix Salviae Miltiorrhizae in high dose group(100mg/kg),middle dose group(30mg/kg),low dose group (10mg/kg).Salvia miltiorrhiza group were administered orally preoperatively,once a day.On 3 consecutive days. Sham operation group and model group were treated with equal volume of saline.The coronary artery ligation rat for 30min,then sonza coronary artery for 180 min to make the model of myocardial ischemia reperfusion injury.Make determination of myocardial infarct size;make determination of serum creatine kinase ( CK ) , lactate dehydrogenase ( LDH) .Results In the model group compared with the sham operation group,myocardial infarction and serum CK and LDH activity significantly increased(t =14.382,21.460,all P<0.05),made up of the myocardial ischemia reperfusion injury in rats model.The different doses of Danshen group can significantly reduce the myocardial ischemia reperfusion injury in rats about the range of myocardial infarction and decrease serum CK , there were significant differences compared with the model group (t=7.426,6.891,11.274,all P<0.05).Different doses of Danshen group can significantly reduce serum LDH, there were significant differences compared with the model group ( t =22.436,10.843,16.252,all P<0.05).Conclusion Salvia miltiorrhiza has oxidative stress on myocardial ischemia reperfusion in rats,which has protective effect of Salvia miltiorrhiza on myocardial ischemia reperfusion injury in rats.