食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2014年
24期
143-148
,共6页
陈科元%陈彦云%贾倩民%任晓月%高媛媛
陳科元%陳彥雲%賈倩民%任曉月%高媛媛
진과원%진언운%가천민%임효월%고원원
马铃薯%贮藏%光照%多胺%萌芽
馬鈴藷%貯藏%光照%多胺%萌芽
마령서%저장%광조%다알%맹아
potato%storage%illumination%polyamine%sprouting
以马铃薯克新1号及青薯168为试供材料，采用二因素完全随机设计，在100%、85%、15%、0%光照强度下贮藏90 d，用高效液相色谱法（RPHPLC）测定不同萌芽状态时（萌芽前、萌芽时、萌芽后）块茎中腐胺、亚精胺和精胺的含量。结果表明：不同光照下两个品种萌芽前多胺含量差异均不显著（P>0.05）；萌芽前克新1号块茎中腐胺和亚精胺均高于青薯168，精胺含量较低，但萌发时克新1号精胺增幅（增加约3~10倍）大于青薯168；不同光照下两个品种的腐胺含量都呈先上升后下降的趋势，萌发时大小顺序均为85%光照>100%光照>15%光照>0%光照，各处理差异显著（P<0.01），腐胺含量越高萌芽越早；萌发后腐胺比萌发前都有所减少，大小顺序为15%光照>0%光照>85%光照>100%光照，各处理差异显著（P<0.01），腐胺含量越高芽越粗；两个品种萌发时亚精胺含量大小顺序均为85%光照>15%光照>100%光照>0%光照，亚精胺含量越高萌芽数越多，随了芽的生长亚精胺都有所增加；两个品种萌发时精胺含量大小顺序与腐胺一致，精胺含量越高萌芽越早，萌发后精胺含量大于萌发前，大小顺序为100%光照>85%光照>15%光照>0%光照，精胺含量越高芽越长。
以馬鈴藷剋新1號及青藷168為試供材料，採用二因素完全隨機設計，在100%、85%、15%、0%光照彊度下貯藏90 d，用高效液相色譜法（RPHPLC）測定不同萌芽狀態時（萌芽前、萌芽時、萌芽後）塊莖中腐胺、亞精胺和精胺的含量。結果錶明：不同光照下兩箇品種萌芽前多胺含量差異均不顯著（P>0.05）；萌芽前剋新1號塊莖中腐胺和亞精胺均高于青藷168，精胺含量較低，但萌髮時剋新1號精胺增幅（增加約3~10倍）大于青藷168；不同光照下兩箇品種的腐胺含量都呈先上升後下降的趨勢，萌髮時大小順序均為85%光照>100%光照>15%光照>0%光照，各處理差異顯著（P<0.01），腐胺含量越高萌芽越早；萌髮後腐胺比萌髮前都有所減少，大小順序為15%光照>0%光照>85%光照>100%光照，各處理差異顯著（P<0.01），腐胺含量越高芽越粗；兩箇品種萌髮時亞精胺含量大小順序均為85%光照>15%光照>100%光照>0%光照，亞精胺含量越高萌芽數越多，隨瞭芽的生長亞精胺都有所增加；兩箇品種萌髮時精胺含量大小順序與腐胺一緻，精胺含量越高萌芽越早，萌髮後精胺含量大于萌髮前，大小順序為100%光照>85%光照>15%光照>0%光照，精胺含量越高芽越長。
이마령서극신1호급청서168위시공재료，채용이인소완전수궤설계，재100%、85%、15%、0%광조강도하저장90 d，용고효액상색보법（RPHPLC）측정불동맹아상태시（맹아전、맹아시、맹아후）괴경중부알、아정알화정알적함량。결과표명：불동광조하량개품충맹아전다알함량차이균불현저（P>0.05）；맹아전극신1호괴경중부알화아정알균고우청서168，정알함량교저，단맹발시극신1호정알증폭（증가약3~10배）대우청서168；불동광조하량개품충적부알함량도정선상승후하강적추세，맹발시대소순서균위85%광조>100%광조>15%광조>0%광조，각처리차이현저（P<0.01），부알함량월고맹아월조；맹발후부알비맹발전도유소감소，대소순서위15%광조>0%광조>85%광조>100%광조，각처리차이현저（P<0.01），부알함량월고아월조；량개품충맹발시아정알함량대소순서균위85%광조>15%광조>100%광조>0%광조，아정알함량월고맹아수월다，수료아적생장아정알도유소증가；량개품충맹발시정알함량대소순서여부알일치，정알함량월고맹아월조，맹발후정알함량대우맹발전，대소순서위100%광조>85%광조>15%광조>0%광조，정알함량월고아월장。
With the Kexin No.1 potato and Qingshu 168 potato original seed as the test for materials , we use two factors completely random design, in 100 %, 85 %,15 %,0 %light illumination store potato 90 d. A highly sensitive and accurate reversed phase high performance liquid chromatographic (RPHPLC) method was developed to determine putrescine, spermidine and spermine of potato tube at different germination status (before sprouting, in the sprouting and after sprouting). The results showed that before sprouting polyamine of two varieties in different storage illumination had no significant difference (P>0.05). Putrescine and spermidine in Kexin No.1 tuber were higher than Qingshu-168 and spermine content was lower, but the content of spermine increase (increased about 3~10 times) larger than Qingshu-168 in the sprouting. Putrescine of two varieties were first increased and then decreased in different storage illumination. Their order of size was 85 %illumination , 100 %illumination, 15 %illumination and 0%illumination, they had significant differences (P<0.01)in the sprouting. The more the putrescine produce, the sooner the buds germinate. After sprouting the putrescine was decreased than before sprouting and their order of size was 15 % illumination, 0 % illumination, 85 %illumination and 100 %illumination, they had significant difference(P<0.01). The more the putrescine produce, the thicker the buds grow. In the budding the spermidine's order of size was 15%illumination, 0%illumination, 85 % illumination and 100 % illumination, the more the putrescine produce, the more the buds grow. The content of spermidine increase with buds grow. The spermine's order of size as putrescine , the more the spermine produce, the sooner the buds germinate. After sprouting, the spermine content greater than before sprouting, their order of size is 100 %illumination, 85 %illumination, 15%illumination and 0%illumination. The more the spermine produce, the longer the buds grow.
