CAJ | 학술논문

对生姜抗多种自由基能力进行检测。通过单因素试验和正交试验分析不同辅助处理方式（微波、超声、普通浸提）、溶剂种类、溶剂浓度、料液比、处理时间以及提取温度等对生姜提取物清除自由基能力的影响。结果表明：不同处理的生姜提取物均对2，2-联氮-二（3-乙基-苯并噻唑-6-磺酸）二铵盐自由基（ABTS+·）和1，1-二苯基-2-三硝基苯肼自由基（DPPH·）有明显的清除作用，对羟基自由基（HO·）的清除作用较小，对超氧阴离子自由基（O2-·）几乎没有清除作用。并且有机浸提溶剂对羟基自由基及超氧自由基具有清除作用。生姜提取物对各种自由基清除能力的大小依次为：ABTS+·﹥DPPH·﹥HO·。
대생강항다충자유기능력진행검측。통과단인소시험화정교시험분석불동보조처리방식（미파、초성、보통침제）、용제충류、용제농도、료액비、처리시간이급제취온도등대생강제취물청제자유기능력적영향。결과표명：불동처리적생강제취물균대2，2-련담-이（3-을기-분병새서-6-광산）이안염자유기（ABTS+·）화1，1-이분기-2-삼초기분정자유기（DPPH·）유명현적청제작용，대간기자유기（HO·）적청제작용교소，대초양음리자자유기（O2-·）궤호몰유청제작용。병차유궤침제용제대간기자유기급초양자유기구유청제작용。생강제취물대각충자유기청제능력적대소의차위：ABTS+·﹥DPPH·﹥HO·。
This paper detects anti-radical ability of ginger. Effects of different adjuvant treatment (microwave extraction, ultrasonic extraction, and traditional extraction), solvent type, solvent concentration, solid-liquid ratio, extraction time and extraction temperature on ginger extractive scavenging radicals were investigated by single factor experiments and orthogonal test. The results showed that each group of ginger extractive had a strong scavenging capacity on 1,1 -Diphenyl -2 -picrylhydrazyl radical (DPPH · )and 2,2' -Azinobis -(3 -ethylbenzthiazoline-6-sulphonate) radical (ABTS+·), but had a small scavenging capacity on hydroxyl radical (HO·). Nevertheless, almost no scavenging activity against superoxide radical (O2-·)was observed. Organic solvent had scavenging capacity on hydroxyl radical and superoxide radical. Ginger extractive on scavenging radicals activity with orders were:ABTS+·﹥DPPH·﹥HO·.