CAJ | 학술논문

以长白山核桃楸种仁分离蛋白为原料，采用Box-Benhnken响应面法对影响还原能力的主要因素pH、温度、加酶量和底物浓度进行研究。确定其最佳工艺参数为：pH7.6，酶解温度50℃，加酶量4510 U/g，底物浓度4.6%，时间4 h，该条件下其还原能力为0.35，水解度为16.50%。抗氧化活性试验表明，随着分离蛋白酶解产物浓度的增加，对DPPH、ABTS、·OH的清除作用及还原能力逐渐增加。当酶解产物浓度达到0.8 mg/mL时，对ABTS的清除率为VC的100%，当浓度达到1.2 mg/mL时，对DPPH的清除率达到相同浓度VC的90.08%。浓度继续增加到15 mg/mL时，对·OH的清除作用为VC的94.56%；这说明优化后的核桃楸种仁分离蛋白酶解产物对DPPH和ABTS具有较强的清除作用，对·OH的清除作用比VC弱。
이장백산핵도추충인분리단백위원료，채용Box-Benhnken향응면법대영향환원능력적주요인소pH、온도、가매량화저물농도진행연구。학정기최가공예삼수위：pH7.6，매해온도50℃，가매량4510 U/g，저물농도4.6%，시간4 h，해조건하기환원능력위0.35，수해도위16.50%。항양화활성시험표명，수착분리단백매해산물농도적증가，대DPPH、ABTS、·OH적청제작용급환원능력축점증가。당매해산물농도체도0.8 mg/mL시，대ABTS적청제솔위VC적100%，당농도체도1.2 mg/mL시，대DPPH적청제솔체도상동농도VC적90.08%。농도계속증가도15 mg/mL시，대·OH적청제작용위VC적94.56%；저설명우화후적핵도추충인분리단백매해산물대DPPH화ABTS구유교강적청제작용，대·OH적청제작용비VC약。
With protein isolate from Juglans mandshurica Maxim kernel in Changbai mountain as the raw material,response surface methodology was applied to optimize the hydrolysis conditions (including pH, temperature, enzyme dosage, and substrate concentration) with reducing power ability as index. The optimum extraction conditions are as follows: pH value of 7. 6, substrate concentration of 4. 6 %, enzyme dosage of 4 510 U/g, enzymolysis temperature of 50℃, and time of 4 h. On this condition, the reducing power ability was 0. 35, the degree of hydrolysis was 16. 50%. The results of antioxidant tests showed that scavenging activity on DPPH, ABTS,·OH and reducing power ability increased with increasing enzymatic hydrolysate concentration. The scavenging activity of enzymatic hydrolysate on DPPH and ABTS was 90. 08%and 100%of VC at 1. 2 mg/mL and 0. 8 mg/mL, the scavenging activity on ·OH was 94. 56%of VC at 15 mg/mL. These demonstrated that the scavenging activity of enzymatic hydrolysate on DPPH and ABTS are strong, the scavenging activity on·OH are weaker than those of VC.