陕西科技大学学报(自然科学版)
陝西科技大學學報(自然科學版)
협서과기대학학보(자연과학판)
JOURNAL OF SHAANXI UNIVERSITY OF SCIENCE & TECHNOLOGY
2015年
1期
126-130
,共5页
甜荞麦皮粉%蛋白质%碱法%盐法%提取
甜蕎麥皮粉%蛋白質%堿法%鹽法%提取
첨교맥피분%단백질%감법%염법%제취
buckwheat bran%protein%alkaline hydrolysis%salt solvent method%extraction
以甜荞麦麸皮为原料,分别以碱法和盐法提取蛋白质.通过单因素实验和正交试验,探讨了不同因素对蛋白质提取率的影响,确定了最佳提取参数.结果表明,碱法和盐法提取蛋白质的等电点分别为3.8和4.2.碱法提取蛋白质的最优条件为:pH10.5,料液比1∶25,温度45℃,时间4h,在此条件下提取率高达70.5%.盐法提取蛋白质的最优条件为:pH7.8,NaCl浓度0.3mol/L,料液比1∶20,温度45℃,时间1.5h,此条件下的提取率高达57.3%.
以甜蕎麥麩皮為原料,分彆以堿法和鹽法提取蛋白質.通過單因素實驗和正交試驗,探討瞭不同因素對蛋白質提取率的影響,確定瞭最佳提取參數.結果錶明,堿法和鹽法提取蛋白質的等電點分彆為3.8和4.2.堿法提取蛋白質的最優條件為:pH10.5,料液比1∶25,溫度45℃,時間4h,在此條件下提取率高達70.5%.鹽法提取蛋白質的最優條件為:pH7.8,NaCl濃度0.3mol/L,料液比1∶20,溫度45℃,時間1.5h,此條件下的提取率高達57.3%.
이첨교맥부피위원료,분별이감법화염법제취단백질.통과단인소실험화정교시험,탐토료불동인소대단백질제취솔적영향,학정료최가제취삼수.결과표명,감법화염법제취단백질적등전점분별위3.8화4.2.감법제취단백질적최우조건위:pH10.5,료액비1∶25,온도45℃,시간4h,재차조건하제취솔고체70.5%.염법제취단백질적최우조건위:pH7.8,NaCl농도0.3mol/L,료액비1∶20,온도45℃,시간1.5h,차조건하적제취솔고체57.3%.
Protein was extracted from buckwheat bran by alkaline hydroiysis and salt solvent method .The effects of influence factors were discussed and the parameters were optimized by single‐factor test and orthogonal designed experiments .The results show :the protein isoe‐lectric point (PI) in alkaline solution and salt solution is respectively 3 .8 and 4 .2 .T he opti‐mal extraction parameters of alkaline hydroiysis were:pH 10 .5 ,solid liquid ratio 1∶25 ,ex‐traction temperature 45 ℃ ,extraction time 4 h ,the protein yield under the optimum condi‐tion is 70 .5% .The optimal extraction parameters of salt solvent method were:pH 7 .8 ,NaCl concentration 0 .3 mol/L ,solid liquid ratio 1∶20 ,extraction temperature 45 ℃ ,extraction time 1 .5 h ,the protein yield under the optimum condition is 57 .30% .