CAJ | 학술논문

建立基于光纤传感技术结合微顺序注射_阀上实验室荧光猝灭测定肠灌流液中H2 S含量方法。本研究进行单因素考察,确定以100μL 0.1mol/L NaOH溶液为载液,荧光素汞浓度为5.0×10-5 mol/L、体积50μL、进样体积50μL、流通池检测流速25μL/s,根据H2 S猝灭荧光素汞521 nm处荧光,对样品中H2 S浓度进行测定。本方法检测H2S的浓度范围为5.0×10-6~8.0×10-5 mol/L,检出限为5.4×10-7 mol/L。测定肠灌流液中H2S含量为3.8×10-5 mol/L,RSD=3.1%(n=3)。本方法能有效在线测定样品中H2S含量,为实时在线测定生物样品中的H2 S含量奠定基础。
건립기우광섬전감기술결합미순서주사_벌상실험실형광졸멸측정장관류액중H2 S함량방법。본연구진행단인소고찰,학정이100μL 0.1mol/L NaOH용액위재액,형광소홍농도위5.0×10-5 mol/L、체적50μL、진양체적50μL、류통지검측류속25μL/s,근거H2 S졸멸형광소홍521 nm처형광,대양품중H2 S농도진행측정。본방법검측H2S적농도범위위5.0×10-6~8.0×10-5 mol/L,검출한위5.4×10-7 mol/L。측정장관류액중H2S함량위3.8×10-5 mol/L,RSD=3.1%(n=3)。본방법능유효재선측정양품중H2S함량,위실시재선측정생물양품중적H2 S함량전정기출。
A fluorescence quenching method was established for the determination of H2 S in intestinal perfusate by optical fiber sensing technology combined with micro sequential injection lab_on_valve (μSIA_lov). In the experiment, 100 μL of 0. 1 mol/L NaOH was used as the carrier, and 50 μL of 5. 0×10-5 mol/L fluorescein mercury and 50 μL of sample were selected for the determination. The detection flow rate at the flowcell was 25 μL/s. According to H2 S quenching fluorescence at 521 nm, the concentration of H2 S in the sample was determined. The detected concentration range of H2S was 5. 0×10-6-8. 0×10-5 mol/L, and the detection limit was 5. 4×10-7 mol/L. Detection result of H2S in intestinal perfusion was 3. 8×10-5 mol/L with 3. 1% RSD (n=3). This method can be used for the determination of H2S effectively in the samples, which lay the foundation for real_time online measurement of H2 S in biological samples.