CAJ | 학술논문

建立了超高效合相色谱法( Ultra performance convergence chromatography,UPC2)分离和测定复合维生素片中11种脂溶性维生素( A, D, E, K)及其衍生物的方法。超高效合相色谱( UPC2)技术集合超临界流体色谱( Supercritical fluid chromatography, SFC )和超高效液相色谱( Ultra performance liquid chromatography, UPLCTM )的技术优点,流动相以CO2为主体,乙腈为助溶剂梯度洗脱。选用Waters Acquity UPC2 HSS C18 SB色谱柱(100 mm ×3.0 mm 1.8μm),流速1 mL/min,检测波长为284 nm。方法检出限在1.5~2.0 mg/L之间；VK1, VK2, VK3和VD3的线性范围分别为3~300 mg/L；VA、VA棕榈酸酯、VA甲酸、VE、VE醋酸酯、VE琥珀酸酯和VD2的线性范围分别为5~300 mg/L；加标回收率范围为97.31%~98.76%；相对标准偏差为0.41%~0.96%,可以满足复合维生素片中11种脂溶性维生素(A, D, E, K)及其衍生物的方法要求。
건립료초고효합상색보법( Ultra performance convergence chromatography,UPC2)분리화측정복합유생소편중11충지용성유생소( A, D, E, K)급기연생물적방법。초고효합상색보( UPC2)기술집합초림계류체색보( Supercritical fluid chromatography, SFC )화초고효액상색보( Ultra performance liquid chromatography, UPLCTM )적기술우점,류동상이CO2위주체,을정위조용제제도세탈。선용Waters Acquity UPC2 HSS C18 SB색보주(100 mm ×3.0 mm 1.8μm),류속1 mL/min,검측파장위284 nm。방법검출한재1.5~2.0 mg/L지간；VK1, VK2, VK3화VD3적선성범위분별위3~300 mg/L；VA、VA종려산지、VA갑산、VE、VE작산지、VE호박산지화VD2적선성범위분별위5~300 mg/L；가표회수솔범위위97.31%~98.76%；상대표준편차위0.41%~0.96%,가이만족복합유생소편중11충지용성유생소(A, D, E, K)급기연생물적방법요구。
A new method was developed for the determination of 11 fat_soluble vitamins ( A, D, E and K) and its derivatives in vitamin tablets by ultra performance convergence chromatography ( UPC2 ) . The mobile phase was the mixture of supercritical CO2 and acetonitrile at a flow rate of 1 mL/min. The separation was carried out on the Waters Acquity UPC2 HSS C18 SB 100 mm × 3. 0 mm i. d. , 1. 8 μm column. The UV detector was set at a wavelength of 284 nm. The limits of detection ( LOD) were 1. 5-2. 0 mg/L, and the calibration linear for VK1 , VK2 , VK3 and VB3 was 3-300 mg/L, linear for VA, VA palmitate, VA formic acid, VE, VE acetate, VD2 and VD3 was 5-300 mg/L, respectively. Its spiked recoveries were 97. 31%-98. 76%, and the relative standard deviations ( RSDs) were 0. 41%-0. 96%. The method is applicable for the determination of fat_soluble vitamins ( A, D, E and K) and Their derivatives in vitamin tablets.