CAJ | 학술논문

以壬基酚( NP)、双酚A( BPA)为模板分子, a_甲基丙烯酸( MAA)为功能单体,通过乳液聚合法制备了双模板分子印迹聚合物( D_MIP),并以D_MIP为固相萃取填料,建立了双分子印迹固相萃取_高效液相色谱荧光检测环境食品中痕量NP和BPA的方法。采用红外光谱和吸附实验对其性能进行了研究。结果表明, D_MIP对NP和BPA的饱和吸附量分别为73.3和97.5 mg/g,相对选择性系数分别为2.2和1.7。在最佳条件下,本方法的线性范围为0.01~2.3 mg/L (R2>0.998),检出限(S/N=3)为0.001~0.002 mg/L。将本方法应用于江水、啤酒、鲫鱼中 NP 和 BPA 含量测定,回收率在86.4%~99.1%范围内,相对标准偏差小于6.2%。本方法选择性好、灵敏度高,对环境和食品中NP和BPA的富集、分离显示出良好的应用前景。
이임기분( NP)、쌍분A( BPA)위모판분자, a_갑기병희산( MAA)위공능단체,통과유액취합법제비료쌍모판분자인적취합물( D_MIP),병이D_MIP위고상췌취전료,건립료쌍분자인적고상췌취_고효액상색보형광검측배경식품중흔량NP화BPA적방법。채용홍외광보화흡부실험대기성능진행료연구。결과표명, D_MIP대NP화BPA적포화흡부량분별위73.3화97.5 mg/g,상대선택성계수분별위2.2화1.7。재최가조건하,본방법적선성범위위0.01~2.3 mg/L (R2>0.998),검출한(S/N=3)위0.001~0.002 mg/L。장본방법응용우강수、비주、즉어중 NP 화 BPA 함량측정,회수솔재86.4%~99.1%범위내,상대표준편차소우6.2%。본방법선택성호、령민도고,대배경화식품중NP화BPA적부집、분리현시출량호적응용전경。
Double template molecularly imprinted polymer ( D_MIP) was prepared by emulsion polymerization method using the nonylphenol (NP), bisphenol A (BPA) as templates, α_methyl methacrylate(MAA) as functional monomer. Then using the D_MIP as solid phase extraction materials, a method based on double_molecularly imprinted solid phase extraction coupled with high performance liquid chromatographic fluorescence detection was developed for the determination of trace NP and BPA from environmental and food samples. The D_MIP was characterized by Fourier transform infrared spectroscopy ( FT_IR ) and adsorption experiments. The results showed that the saturated adsorption capacity of the D_MIP for NP and BPA was 73. 3 and 97. 5 mg/g, respectively, and the relative selectivity coefficient was 2. 2 and 1. 7, respectively. Moreover, the conditions affecting extraction efficiency, such as washing conditions and desorption conditions were optimized. Under the optimal conditions, there was a good linear correlation in the concentration range of 0. 01-2. 3 mg/L with correlation coefficients (R2) greater than 0. 998 for NP and BPA. The detection limits ( S/N=3) were 0. 001 and 0. 002 mg/L. The proposed method was applied to determine NP and BPA in river water, beer and crucian carp samples. The recoveries of NP and BPA were in the range of 86. 4%-99. 1%with the relative standard deviation less than 6 . 2%. This method has good selectivity and high sensitivity and shows good prospects for the application in the enrichment and separation of NP and BPA in real samples.