CAJ | 학술논문

为了实现对微弱神经递质信号的在体实时检测，设计并实现了一种无线电化学检测仪器。系统硬件以低功耗微控制器MSP430为主控制器，包括了微弱电流检测模块、波形产生模块以及数据收发模块，具有尺寸小(2.3 cm×1.8 cm×0.6 cm)，功耗低的特点。基于uC/OS操作系统设计了系统的下位机程序，结合上位机软件实现了检测数据的实时显示与分析。针对神经递质的实际检测需求，系统集成了计时电流法与快速循环伏安法两种电化学检测分析方法。利用快速循环伏安法对不同浓度的多巴胺标准溶液进行了测试，在浓度范围5.0×10-7~7.0×10-5 mol/L内，系统检测的响应电流与多巴胺浓度之间线性相关系数R=0.99。在此基础上，开展了大鼠在体多巴胺检测的实验，并成功检测到大鼠尾状壳核脑区的电诱发多巴胺释放信号。实验结果表明，此仪器具有检测灵敏度高的优点，能够实现对大脑内神经递质信号的定性及定量分析，在神经科学研究领域具有广阔的应用前景。
위료실현대미약신경체질신호적재체실시검측，설계병실현료일충무선전화학검측의기。계통경건이저공모미공제기MSP430위주공제기，포괄료미약전류검측모괴、파형산생모괴이급수거수발모괴，구유척촌소(2.3 cm×1.8 cm×0.6 cm)，공모저적특점。기우uC/OS조작계통설계료계통적하위궤정서，결합상위궤연건실현료검측수거적실시현시여분석。침대신경체질적실제검측수구，계통집성료계시전류법여쾌속순배복안법량충전화학검측분석방법。이용쾌속순배복안법대불동농도적다파알표준용액진행료측시，재농도범위5.0×10-7~7.0×10-5 mol/L내，계통검측적향응전류여다파알농도지간선성상관계수R=0.99。재차기출상，개전료대서재체다파알검측적실험，병성공검측도대서미상각핵뇌구적전유발다파알석방신호。실험결과표명，차의기구유검측령민도고적우점，능구실현대대뇌내신경체질신호적정성급정량분석，재신경과학연구영역구유엄활적응용전경。
A wireless electrochemical recording device was designed for in_vivo neurotransmitters real_time detection. Low_power microcontroller MSP430 was chosen as main control unit in hardware system. Other modules were current detection module, waveform generator module and data transceiver module. This device had the merits of small size (2. 3 cm×1. 8 cm×0. 6 cm) and low power consumption. Firmware program design was based on uC/OS operating system. Combined with the PC software, the device could achieve online display and analysis of the recording data. For neurotransmitter detecting needs, the device implemented fast_scan cyclic voltammetry ( FSCV) and fixed_potential amperometry. By using fast_scan cyclic voltammetry method, a linear relationship ( R=0. 99 ) between the concentration of dopamine and response current was acquired in the range of 5. 0×10-7-7. 0×10-5 mol/L. In the in_vivo experiments, the electrically evoked dopamine was recorded in the caudate_putamen area of brain in rats. Experimental results showed that the system had high detection accuracy, which could realize qualitative and quantitative analysis of the brain neurotransmitter. This work would have a broad application prospect in the field of neuroscience research.