CAJ | 학술논문

婴儿的胎发是评价婴儿在母体环境中污染物累积暴露水平的良好介质。含氯持久性有机污染物是人体中残留浓度最高的持久性有机污染物。本研究依次采用HCl酸化、正己烷/二氯甲烷混合溶液萃取、硅胶和氧化铝混合填充小柱净化,以及气相色谱高分辨质谱法检测了北京20个婴儿胎发中的16种典型含氯持久性有机污染物。结果表明,16种目标物的检出限可达到1.0~2.5μg/kg,替代物五氯硝基苯回收率大于67.6%,加标样品中目标物回收率范围为62.5%~92.3%。20个胎发样品中六氯苯、β_六六六和p,pˊ_滴滴伊检出率均为100%,γ_六六六、2,4,4ˊ_三氯联苯和2,2ˊ,5,5ˊ_四氯联苯的检出率分别为85%,50%和40%。4种污染物总量的浓度分布范围分别为5.48~8.40μg/kg(六氯苯)、3.86~27.1μg/kg (六六六)、1.16~18.3μg/kg(滴滴涕)和2.20~22.1μg/kg(多氯联苯),平均浓度分别为6.91,6.10,5.53和3.44μg/kg。此方法和分析结果可用于胎儿体内16种含氯持续性有机污染物蓄积水平和母体内累计暴露水平的评价。
영인적태발시평개영인재모체배경중오염물루적폭로수평적량호개질。함록지구성유궤오염물시인체중잔류농도최고적지구성유궤오염물。본연구의차채용HCl산화、정기완/이록갑완혼합용액췌취、규효화양화려혼합전충소주정화,이급기상색보고분변질보법검측료북경20개영인태발중적16충전형함록지구성유궤오염물。결과표명,16충목표물적검출한가체도1.0~2.5μg/kg,체대물오록초기분회수솔대우67.6%,가표양품중목표물회수솔범위위62.5%~92.3%。20개태발양품중륙록분、β_륙륙륙화p,pˊ_적적이검출솔균위100%,γ_륙륙륙、2,4,4ˊ_삼록련분화2,2ˊ,5,5ˊ_사록련분적검출솔분별위85%,50%화40%。4충오염물총량적농도분포범위분별위5.48~8.40μg/kg(륙록분)、3.86~27.1μg/kg (륙륙륙)、1.16~18.3μg/kg(적적체)화2.20~22.1μg/kg(다록련분),평균농도분별위6.91,6.10,5.53화3.44μg/kg。차방법화분석결과가용우태인체내16충함록지속성유궤오염물축적수평화모체내루계폭로수평적평개。
Infant hair is a good medium to assess the cumulative exposure level of infant in the mother ' s body. Chlorinated persistent organic pollutants were ubiquitous and with the highest volume concentration in the human body. In this experiment, 20 infant hairs were collected from Beijing. The sample was overnight incubated in HCl, extracted with the mixture of hexane and dichloromethane, and cleaned up by a cartridge filled with alumina and acidified silica. The final eluate was concentrated, and 16 kinds of typical chlorinated persistent organic pollutants were detected with gas chromatography_high resolution mass spectrometry ( HRGC_MS). The results showed that the detection limit of the target compounds in sample was 1. 00-2. 50 μg/kg, the recovery of surrogate in all samples was more than 67 . 6%, and the range of recoveries for target compounds in spiked sample was 62. 5%-92. 3%. The positive rate is 100% for hexachlorobenzene,β_HCH and p, pˊ_DDE, 85% for γ_HCH, 50% for PCB28, and 40% for PCB52. The concentrations of Hexachlorobenzene, HCHs, DDTs and PCBs were 5. 48-8. 40 μg/kg, 3. 86-27. 1 μg/kg, 1. 16-18. 3 μg/kg and 2. 20-22. 1 μg/kg, respectively. The average concentrations were 7. 84 μg/kg for hexachlorobenzene, 6. 93 μg/kg for HCHs, 5. 53 μg/kg for DDTs, and 3. 44 μg/kg for PCBs. The method and the analysis results can be used to evaluate the accumulation level and cumulative exposure level of 16 target compounds for fetal in motherˊs body.