CAJ | 학술논문

本研究通过超声提取_固相萃取,建立了利用超高效液相色谱/串联质谱同时分析粪便、土壤和水体中25种兽药抗生素的方法,考察了串联SAX小柱对土壤样品和粪便样品的净化效果,以及正己烷提取对粪便样品提取液脱脂的净化效果。实验结果表明：本方法对25种兽药抗生素加标回收率为50.0%~121.9%,相对标准偏差为1.1%~14.7%(n=9)；土壤和粪便的方法检出限为0.0002~0.0560μg/kg,水体的方法检出限为0.002~0.28 ng/L；串联SAX强阴离子交换柱后,土壤样品的基质效应降低为75%~160%之间,粪便样品降低为55%~120%之间；粪便样品经正己烷脱脂后,基质效降为55%~120%之间。已将本方法应用于养殖场周边环境介质的检测。
본연구통과초성제취_고상췌취,건립료이용초고효액상색보/천련질보동시분석분편、토양화수체중25충수약항생소적방법,고찰료천련SAX소주대토양양품화분편양품적정화효과,이급정기완제취대분편양품제취액탈지적정화효과。실험결과표명：본방법대25충수약항생소가표회수솔위50.0%~121.9%,상대표준편차위1.1%~14.7%(n=9)；토양화분편적방법검출한위0.0002~0.0560μg/kg,수체적방법검출한위0.002~0.28 ng/L；천련SAX강음리자교환주후,토양양품적기질효응강저위75%~160%지간,분편양품강저위55%~120%지간；분편양품경정기완탈지후,기질효강위55%~120%지간。이장본방법응용우양식장주변배경개질적검측。
A liquid chromatography_tandem mass spectrometric method was established for the determination of 25 kinds of veterinary antibiotics in manure, soil and water by ultrasonic_solid phase extraction. Purification efficiency of SAX cartridges for soil and manure samples extract and the procedure that manure samples defatted with hexane was also verified. The results showed that the average recoveries of the 25 target antibiotics were 50. 0%-121. 9%, the relative standard deviations (RSD) were 1. 1%-14. 71% (n=9), and the limits of detection (LOD) ranged from 0. 0002-0. 0560 μg/kg for soil and manure and ranged from 0. 002-0. 28 ng/L for water; After adding SAX cartridges, the matrix effects of manure samples reduced to 55%-120%, and soil samples to 75%-160%; after defatting by hexane extraction, the matrix effects of manure sample reduced to 55%-120%. This method has been employed to detect the veterinary antibiotics in environmental samples of livestock farm.