CAJ | 학술논문

利用基于气相色谱_质谱联用技术( GC_MS)的代谢组学方法，寻找不同处理条件间差异显著的镉超富集植物东南景天根系分泌物质，并探讨它们对东南景天耐受或超富集镉的可能作用机制。收集0和40μmol/L镉分别处理4和8天后的东南景天根系分泌物样品，通过样品冻干、甲醇溶解、甲氧胺盐酸盐和N_甲基_N_(三甲基硅烷)三氟乙酰胺衍生化处理、GC_MS检测的分析过程，得到根系分泌物的表达谱。主成分分析和正交偏最小二乘判别分析( OPLS_DA)得分图可将不同处理条件间东南景天的根系分泌物质明显区分，运用OPLS_DA载荷图、模型的变量重要性因子和方差分析发现12个根系分泌物质在4组间存在显著性差异。它们的相对含量在不同处理条件间的变化趋势明显不同，表明东南景天可通过调节它们的分泌来耐受或超富集重金属镉。
이용기우기상색보_질보련용기술( GC_MS)적대사조학방법，심조불동처리조건간차이현저적력초부집식물동남경천근계분비물질，병탐토타문대동남경천내수혹초부집력적가능작용궤제。수집0화40μmol/L력분별처리4화8천후적동남경천근계분비물양품，통과양품동간、갑순용해、갑양알염산염화N_갑기_N_(삼갑기규완)삼불을선알연생화처리、GC_MS검측적분석과정，득도근계분비물적표체보。주성분분석화정교편최소이승판별분석( OPLS_DA)득분도가장불동처리조건간동남경천적근계분비물질명현구분，운용OPLS_DA재하도、모형적변량중요성인자화방차분석발현12개근계분비물질재4조간존재현저성차이。타문적상대함량재불동처리조건간적변화추세명현불동，표명동남경천가통과조절타문적분비래내수혹초부집중금속력。
A metabonomics method based on gas chromatography_mass spectrometry ( GC_MS) was developed for detecting the significant differences of root exudates of the Cd hyperaccumulator Sedum alfredii under different treatments and study the effect mechanism of S. alfredii tolerating or accumulating the heavy metal Cd. The root exudates were collected after treatment for 4 and 8 days with 0 and 40μmol/L Cd. The collected solution was lyophilized and dissolved with methanol, and after derivatization with methoxyamine hydrochloride and N_methyl_N_trifluoroacetamide, the samples were analyzed by GC_MS. Principal component analysis ( PCA) and orthogonal partial least_squares discrimination analysis ( OPLS_DA) were carried out for pattern recognition and a clear separation among the different treatments was achieved. Twelve compounds which caused the separation among the different treatments were found and identified. With the change of treatments, the relative amount of these 12 compounds revealed different trends, which indicated that the Cd hyperaccumulator S. alfredii could adjust the secretion of root exudates to tolerate or accumulate the heavy metal Cd.