中国蔬菜
中國蔬菜
중국소채
CHINA VEGETABLES
2014年
12期
21-26
,共6页
刘东明%杨丽梅%唐俊%刘泽洲%方智远%刘玉梅%庄木%张扬勇%孙培田%李景涛
劉東明%楊麗梅%唐俊%劉澤洲%方智遠%劉玉梅%莊木%張颺勇%孫培田%李景濤
류동명%양려매%당준%류택주%방지원%류옥매%장목%장양용%손배전%리경도
结球甘蓝%无蜡粉亮绿%特征特性%遗传规律%分子标记
結毬甘藍%無蠟粉亮綠%特徵特性%遺傳規律%分子標記
결구감람%무사분량록%특정특성%유전규률%분자표기
Cabbage%Glossy wax-less%Characteristics%Inheritance%Molecular marker
为了明确结球甘蓝无蜡粉亮绿突变体材料LD10的特征特性及遗传规律，测定了LD10的主要农艺性状和营养物质含量；对LD10和普通材料21-3的叶面超微结构进行了观察比较；以LD10和21-3为亲本构建6世代群体，对LD10无蜡粉亮绿性状遗传规律进行分析。结果表明：无蜡粉亮绿突变体材料LD10叶面蜡粉呈小颗粒状结构，缺失严重，而普通材料21-3叶面蜡粉呈长棒状结构，含量丰富，二者具有明显差异；LD10的无蜡粉亮绿性状由1个隐性基因（cgl-4）控制，其与SSR标记LT-SSR16紧密连锁，遗传距离为4.7 cM。用LD10与另一单基因隐性遗传的无蜡粉亮绿突变体材料10Q-961杂交获得F1群体，F1群体单株全部表现为叶面有蜡粉，推断LD10与10Q-961的无蜡粉亮绿基因位于不同的遗传位点。
為瞭明確結毬甘藍無蠟粉亮綠突變體材料LD10的特徵特性及遺傳規律，測定瞭LD10的主要農藝性狀和營養物質含量；對LD10和普通材料21-3的葉麵超微結構進行瞭觀察比較；以LD10和21-3為親本構建6世代群體，對LD10無蠟粉亮綠性狀遺傳規律進行分析。結果錶明：無蠟粉亮綠突變體材料LD10葉麵蠟粉呈小顆粒狀結構，缺失嚴重，而普通材料21-3葉麵蠟粉呈長棒狀結構，含量豐富，二者具有明顯差異；LD10的無蠟粉亮綠性狀由1箇隱性基因（cgl-4）控製，其與SSR標記LT-SSR16緊密連鎖，遺傳距離為4.7 cM。用LD10與另一單基因隱性遺傳的無蠟粉亮綠突變體材料10Q-961雜交穫得F1群體，F1群體單株全部錶現為葉麵有蠟粉，推斷LD10與10Q-961的無蠟粉亮綠基因位于不同的遺傳位點。
위료명학결구감람무사분량록돌변체재료LD10적특정특성급유전규률，측정료LD10적주요농예성상화영양물질함량；대LD10화보통재료21-3적협면초미결구진행료관찰비교；이LD10화21-3위친본구건6세대군체，대LD10무사분량록성상유전규률진행분석。결과표명：무사분량록돌변체재료LD10협면사분정소과립상결구，결실엄중，이보통재료21-3협면사분정장봉상결구，함량봉부，이자구유명현차이；LD10적무사분량록성상유1개은성기인（cgl-4）공제，기여SSR표기LT-SSR16긴밀련쇄，유전거리위4.7 cM。용LD10여령일단기인은성유전적무사분량록돌변체재료10Q-961잡교획득F1군체，F1군체단주전부표현위협면유사분，추단LD10여10Q-961적무사분량록기인위우불동적유전위점。
To clarify the characteristics and inheritance of cabbage(Brassica oleracea L. var. capitataL.) glossy wax-less mutant LD10,the major agronomic characteristics and nutrient contents of LD10 were tested, and ultra-structures of leaves in mutant material LD10 and normal material 21-3 were measured,observed and comparatively analyzed. LD10 and 21-3 were taken as parent to construct 6 generations population,and inheritance analysis onthe glossy wax-less characteristics of LD10 was conducted. The results showed that there was little wax on the leaves of LD10,while the wax on the leaves of 21-3 was abundent,and the granular wax in LD10 was of long stick shape,significantly different from the wax in 21-3. The glossy wax-less trait in cabbage LD10 was controlled by one single recessive gene named cgl-4. One molecular marker(LT-SSR16)linked to cgl-4 with genetic distances 4.7 cM. Another F1 population obtained through crossing LD10 and 10Q-961 showed that the genes leading to glossy wax-less trait were at different locations in the two glossy wax-less materials.
