中国蔬菜
中國蔬菜
중국소채
CHINA VEGETABLES
2014年
12期
15-20
,共6页
赵黎明%李刚%刘永光%国家进%魏家鹏%竺晓平
趙黎明%李剛%劉永光%國傢進%魏傢鵬%竺曉平
조려명%리강%류영광%국가진%위가붕%축효평
番茄褪绿病毒%番茄黄化曲叶病毒%RT-PCR检测%复合侵染
番茄褪綠病毒%番茄黃化麯葉病毒%RT-PCR檢測%複閤侵染
번가퇴록병독%번가황화곡협병독%RT-PCR검측%복합침염
Tomato chlorosis virus%Tomato yellow leaf curl virus%RT-PCR detection%Mixed infections
2013年秋季，在山东寿光设施番茄植株上采集到叶片脉间褪绿黄化、叶片边缘卷曲、叶片皱缩，后期叶片变厚、变 脆，并伴随着植株矮化的疑似番茄褪绿病毒和番茄黄化曲叶病毒复合侵染的样本。分别利用番茄褪绿病毒（Tomato chlorosis virus， ToCV）外壳蛋白基因（CP）序列引物CP-F/CP-R和番茄黄化曲叶病毒（Tomato yellow leaf curl virus， TYLCV）特异引物TY1-R/TY1-F、TY2-R/TY2-F、TY3-R/TY3-F对其进行检测、扩增，分别得到774 bp（GenBank登录号KC709510）和2 781 bp（GenBank登录号KJ546418）的核苷酸序列。经序列测序后表明，KC709510与GenBank已登录的番茄褪绿病毒ToCV-BJ（KC311375）分离物相似性为99.6%，KJ546418与GenBank已登录的番茄黄化曲叶病毒TYLCV-SDWF-L7（KC999850）分离物相似性为99.6%。
2013年鞦季，在山東壽光設施番茄植株上採集到葉片脈間褪綠黃化、葉片邊緣捲麯、葉片皺縮，後期葉片變厚、變 脆，併伴隨著植株矮化的疑似番茄褪綠病毒和番茄黃化麯葉病毒複閤侵染的樣本。分彆利用番茄褪綠病毒（Tomato chlorosis virus， ToCV）外殼蛋白基因（CP）序列引物CP-F/CP-R和番茄黃化麯葉病毒（Tomato yellow leaf curl virus， TYLCV）特異引物TY1-R/TY1-F、TY2-R/TY2-F、TY3-R/TY3-F對其進行檢測、擴增，分彆得到774 bp（GenBank登錄號KC709510）和2 781 bp（GenBank登錄號KJ546418）的覈苷痠序列。經序列測序後錶明，KC709510與GenBank已登錄的番茄褪綠病毒ToCV-BJ（KC311375）分離物相似性為99.6%，KJ546418與GenBank已登錄的番茄黃化麯葉病毒TYLCV-SDWF-L7（KC999850）分離物相似性為99.6%。
2013년추계，재산동수광설시번가식주상채집도협편맥간퇴록황화、협편변연권곡、협편추축，후기협편변후、변 취，병반수착식주왜화적의사번가퇴록병독화번가황화곡협병독복합침염적양본。분별이용번가퇴록병독（Tomato chlorosis virus， ToCV）외각단백기인（CP）서렬인물CP-F/CP-R화번가황화곡협병독（Tomato yellow leaf curl virus， TYLCV）특이인물TY1-R/TY1-F、TY2-R/TY2-F、TY3-R/TY3-F대기진행검측、확증，분별득도774 bp（GenBank등록호KC709510）화2 781 bp（GenBank등록호KJ546418）적핵감산서렬。경서렬측서후표명，KC709510여GenBank이등록적번가퇴록병독ToCV-BJ（KC311375）분리물상사성위99.6%，KJ546418여GenBank이등록적번가황화곡협병독TYLCV-SDWF-L7（KC999850）분리물상사성위99.6%。
In the autumn of 2013, leaves of greenhouse-grown tomato plants showed symptoms of interveinal chlorosis, and together with stunted, marginal curling and crumpling. As the disease progressed, leaves become thick and crisp, similar to symptoms induced by Tomato chlorosis virus (ToCV) and Tomato yellow leaf curl virus (TYLCV) were collected from greenhouse-grown tomato plants in Shouguang, Shandong Province. The specific primer pairs of CP-F/CP-R specific to ToCV and TY1-R/TY1-F,TY2-R/TY2-F,TY3-R/TY3-F specific to TYLCV were used to detect ToCV and TYLCV, respectively. 774 bp (GenBank accession number: KC709510) and 2 781 bp (GenBank accession number: KJ546418) specific fragments were amplified from the same symptomatic samples. Sequence analysis of the amplified sequence of KC709510 showed 99.6% nucleotide sequence identity with ToCV-BJ (KC311375) isolates and the sequence of KJ546418 showed 99.6% nucleotide sequence identity with TYLCV-SDWF-L7 (KC999850) isolates registered in the GenBank.
