CAJ | 학술논문

目的：：观察糖尿病视网膜病变大鼠模型视网膜血管的病理变化及VEGF表达变化,初步评价双丹明目胶囊抑制视网膜血管新生效应。方法：将双丹明目胶囊作用于糖尿病视网膜病变大鼠,通过与正常组、模型对照组、阳性对照组的比较,电镜下观察双丹明目胶囊对糖尿病视网膜病变大鼠视网膜组织的影响,HE染色后光镜下观察视网膜结构,并采用免疫组织化学法检测大鼠视网膜中VEGF的表达。结果：经双丹明目胶囊治疗2 mo后,双丹明目组大鼠视网膜组织轻度水肿,毛细血管周细胞水肿,线粒体轻度肿胀,结构稍欠清晰,部分内皮细胞轻度增生。除正常组外各组视网膜VEGF表达增加,以模型对照组最为明显,且双丹明目组和阳性对照组与模型对照组VEGF的表达有明显差异(P<0.01)。结论：双丹明目胶囊能有效地改善糖尿病大鼠视网膜微血管改变,减轻视网膜各层结构水肿、坏死情况,改善超微结构改变,可以抑制DR大鼠视网膜VEGF表达。
목적：：관찰당뇨병시망막병변대서모형시망막혈관적병리변화급VEGF표체변화,초보평개쌍단명목효낭억제시망막혈관신생효응。방법：장쌍단명목효낭작용우당뇨병시망막병변대서,통과여정상조、모형대조조、양성대조조적비교,전경하관찰쌍단명목효낭대당뇨병시망막병변대서시망막조직적영향,HE염색후광경하관찰시망막결구,병채용면역조직화학법검측대서시망막중VEGF적표체。결과：경쌍단명목효낭치료2 mo후,쌍단명목조대서시망막조직경도수종,모세혈관주세포수종,선립체경도종창,결구초흠청석,부분내피세포경도증생。제정상조외각조시망막VEGF표체증가,이모형대조조최위명현,차쌍단명목조화양성대조조여모형대조조VEGF적표체유명현차이(P<0.01)。결론：쌍단명목효낭능유효지개선당뇨병대서시망막미혈관개변,감경시망막각층결구수종、배사정황,개선초미결구개변,가이억제DR대서시망막VEGF표체。
Abstract?AlM: To observe the effects of Shuangdanmingmu capsule on VEGF expression and retinal vascular morphology in rats with diabetic retinopathy ( DR) .?METHODS: DR rats were fed with Shuangdanmingmu capsule. By comparing with the normal group, the model control group, and positive control group, the effect of Shuangdanmingmu capsule on retinal tissue of DR rats was observed under electron microscopy. After HE staining, retinal structure was observed under the light microscope. lmmunohitochemical staining was used to detect the VEGF expression in retina.?RESULTS:Two months after treatment, the layers tissue of retina presented mild edema, capillary pericytes performed edema, mitochondria showed mild swelling and less clear structure, some endothelial cells showed slight proliferation in Shuangdanmingmu group. Compared with the normal group, the expression level of VEGF in retina increased in the other groups, especially in model control group. A significant differential in expression of VEGF was found between Shuangdanmingmu group, positive control group and model control group (P<0. 01).? CONCLUSlON: Shuangdanmingmu capsule can effectively improve the retinal microvascular, reduce edema and necrosis of each layer of retina, improve the ultrastructure of retina's tissue and inhibit VEGF expression in DR rats.