粮食与油脂
糧食與油脂
양식여유지
CEREALS & OILS
2015年
1期
20-23
,共4页
荷叶%黄酮%分离%大孔树脂%抗氧化
荷葉%黃酮%分離%大孔樹脂%抗氧化
하협%황동%분리%대공수지%항양화
lotus leaves%flavonoids%separation%macroporous resins%antioxidant
本试验采用水提法提取荷叶黄酮，按提取温度80℃、料液比1∶20、提取时间为1.5 h条件得到荷叶黄酮提取量为17.33 mg/g。比较X–5、HP20、HPD100、HZ801、HZ818大孔吸附树脂对荷叶黄酮的静态吸附、洗脱性能，确定HPD100为适宜树脂。进一步考察大孔树脂HPD100的动态吸附洗脱能力，研究结果得出大孔树脂HPD100分离荷叶黄酮的适宜工艺参数为：常温下流速为2 BV/h，上样液浓度为0.700 mg/mL上柱吸附，洗脱流速为3 BV/h，体积分数60%乙醇洗脱，用量60 mL。在此工艺条件下，总黄酮得率为73.78%，总黄酮纯度为64.2%。对油脂抗氧化试验结果显示，添加量为1.50 mg荷叶黄酮抗氧化作用比2 mg维生素C好。
本試驗採用水提法提取荷葉黃酮，按提取溫度80℃、料液比1∶20、提取時間為1.5 h條件得到荷葉黃酮提取量為17.33 mg/g。比較X–5、HP20、HPD100、HZ801、HZ818大孔吸附樹脂對荷葉黃酮的靜態吸附、洗脫性能，確定HPD100為適宜樹脂。進一步攷察大孔樹脂HPD100的動態吸附洗脫能力，研究結果得齣大孔樹脂HPD100分離荷葉黃酮的適宜工藝參數為：常溫下流速為2 BV/h，上樣液濃度為0.700 mg/mL上柱吸附，洗脫流速為3 BV/h，體積分數60%乙醇洗脫，用量60 mL。在此工藝條件下，總黃酮得率為73.78%，總黃酮純度為64.2%。對油脂抗氧化試驗結果顯示，添加量為1.50 mg荷葉黃酮抗氧化作用比2 mg維生素C好。
본시험채용수제법제취하협황동，안제취온도80℃、료액비1∶20、제취시간위1.5 h조건득도하협황동제취량위17.33 mg/g。비교X–5、HP20、HPD100、HZ801、HZ818대공흡부수지대하협황동적정태흡부、세탈성능，학정HPD100위괄의수지。진일보고찰대공수지HPD100적동태흡부세탈능력，연구결과득출대공수지HPD100분리하협황동적괄의공예삼수위：상온하류속위2 BV/h，상양액농도위0.700 mg/mL상주흡부，세탈류속위3 BV/h，체적분수60%을순세탈，용량60 mL。재차공예조건하，총황동득솔위73.78%，총황동순도위64.2%。대유지항양화시험결과현시，첨가량위1.50 mg하협황동항양화작용비2 mg유생소C호。
The flavonoids were extracted from lotus leaves with water extraction process in this study. The optimized extraction process conditions were 80 oC of extraction temperature,1∶20 of solid–liquid ratio,and 1.5 h of extracting time with an extraction rate of 17.33 mg/g. The performances of macroporous resins such as X–5,HP20,HPD100,HZ801,HZ818 were evaluated by the adsorption and elution properties of flavonoids. The HPD100 is confirmed to be suitable resin. The dynamic adsorption and elution ability of macroporous resin HPD100 was further investigated. The result indicated that appropriate parameters for separation flavonoids from lotus leaves with HPD100 macroporous resin were 2 BV/h of adsorption velocity,0.700 mg/mL of sample concentration,3 BV/h of elution velocity, 60% of ethanol solution and 60 mL of eluant volume under ambient temperature. Under the above conditions,the yield and purity of total flavonoids is 73.78%and 64.2%,respectively. The oil oxidation resistance test showed that the antioxidant activity of 1.5 mg flavonoids from lotus leaves was higher than that of 2 mg Vitamin C.
