CAJ | 학술논문

目的：研究7例Rh部分D样本的基因分型。方法采用PCR－SSP技术检测常规血清学试验为D变异型的样本中的Rh部分D进行D基因分型。结果在30例常规血清学试验中，23例具有完整的RHD基因外显子（ Rh弱D型），7例部分外显子缺失，为Rh部分D，23例为弱D型，并对部分D样本进行了基因分型，4例检测为D Cat．ⅥⅢ，1例为D Cat．Ⅵtype2，1例为D Cat．Ⅵtype 1，1例为基因2？．9外显子融合，表示为RHD－CE（2－9）－D。结论Rh部分D型与弱D型在血清学试验中无法区分，采用分子生物学方法才能够更准确的分型，更能确保输血安全。
목적：연구7례Rh부분D양본적기인분형。방법채용PCR－SSP기술검측상규혈청학시험위D변이형적양본중적Rh부분D진행D기인분형。결과재30례상규혈청학시험중，23례구유완정적RHD기인외현자（ Rh약D형），7례부분외현자결실，위Rh부분D，23례위약D형，병대부분D양본진행료기인분형，4례검측위D Cat．ⅥⅢ，1례위D Cat．Ⅵtype2，1례위D Cat．Ⅵtype 1，1례위기인2？．9외현자융합，표시위RHD－CE（2－9）－D。결론Rh부분D형여약D형재혈청학시험중무법구분，채용분자생물학방법재능구경준학적분형，경능학보수혈안전。
Objective To investigate genotyping of 7 cases of partial RhD specimens .Methods The genetyping technique ( PCR-SSP ) was performed for partial RhD variants identified by common serological tests .Results Among 30 specimens identified as D variants during routine serological experiment ,7 specimens were defined as partial RhD and 23 specimens as weak D .Then 7 specimens of partial RhD were genetyped further in which 4 cases were D Cat .ⅥⅢ,1 case was D Cat.Ⅵ type 2,1cases was D Cat.Ⅵ type 1 and 1 case was RHD-CE(2-9)-D.Conclusion The routine serological experiments can not differentiate partial D from weak D ,however ,the molecular biological methods can type accurately in order to ensure the safety of blood transfusion .