CAJ | 학술논문

目的：研究灵芝孢子粉对人卵巢癌OV2008细胞增殖及凋亡的影响，并探讨其作用机制。方法采用WST－1比色法及显微镜观察法研究灵芝孢子粉对OV2008细胞生长抑制作用及细胞形态的改变；流式细胞仪检测细胞周期分布情况；Hoechst－33258染色方法评估细胞凋亡并采用western blot 方法测定凋亡相关蛋白的表达。结果WST结果表明灵芝孢子粉可以抑制OV2008细胞的生长，并呈剂量和时间依赖性；流式细胞术结果提示灵芝孢子粉可使卵巢癌细胞生长阻滞于G1期。 Hoechst－33258染色结果显示灵芝孢子处理细胞后可以检测出凋亡细胞。 Western blot方法检测到凋亡抑制蛋白bcl－2、bcl－xl随灵芝孢子浓度增加而下降，而促凋亡蛋白bax及P27、casep具有抑制卵巢癌细胞生长及诱导凋亡的作用，其机制是作用于细胞周期的ase－3逐渐升高。结论灵芝孢子粉可以抑制卵巢细胞生长，使细胞生长阻滞于G1期，并可以通过上调bax、P27，下调bcl－2并活化casepase－3而发挥其作用。
목적：연구령지포자분대인란소암OV2008세포증식급조망적영향，병탐토기작용궤제。방법채용WST－1비색법급현미경관찰법연구령지포자분대OV2008세포생장억제작용급세포형태적개변；류식세포의검측세포주기분포정황；Hoechst－33258염색방법평고세포조망병채용western blot 방법측정조망상관단백적표체。결과WST결과표명령지포자분가이억제OV2008세포적생장，병정제량화시간의뢰성；류식세포술결과제시령지포자분가사란소암세포생장조체우G1기。 Hoechst－33258염색결과현시령지포자처리세포후가이검측출조망세포。 Western blot방법검측도조망억제단백bcl－2、bcl－xl수령지포자농도증가이하강，이촉조망단백bax급P27、casep구유억제란소암세포생장급유도조망적작용，기궤제시작용우세포주기적ase－3축점승고。결론령지포자분가이억제란소세포생장，사세포생장조체우G1기，병가이통과상조bax、P27，하조bcl－2병활화casepase－3이발휘기작용。
Objective To investigate the effect of Ganoderma lucidum spore powder ( GLSP) on cell proliferation and apoptosis of human ovarian cell line-OV 2008,and to explore its action mechanism .Methods The effects of GLSP on OV2008 cell growth and cell morphous were detected by WST-1 colorimetric technique and microscopy; cell cycle was analyzed flow cytometry;cell apoptosis was detected by Hoechst-33258 staining method;the expression of apoptosis-related proteins ( bcl-2 bax,P27,cleaved Caspase-3) were measured by Western blotting .Results The results of WST-1 assays showed that GLSP could inhibit the growth of OV2008 cells,moreover,which was time and dose dependent .The results by flow cytometry showed that GLSP could block OV2008 cell growth at G1 phase.Hoechst 33258 staining results showed that GLSP could induce cell apoptosis.Western blotting results showed that the expression levels of bcl-2, bcl-xl were decreased with the increase of concentration of GLSP,however bax,P27,Caspase-3 could inhibit the growth of ovarian cancer cells and induce cell apoptosis , and its action mechanism was that the expression levels of Casbase-3 that affected cell cycle were gradually increased . Conclusion GLSP can inhibit the growth of ovarian cancer cells , block cell growth at G1 stage, furthermore, which can develop its effects by up-regulating the expression of bax , P27, down-regulating the expression of bcl-2 and activating Caspase-3.