亚热带植物科学
亞熱帶植物科學
아열대식물과학
SUBTROPICAL PLANT SCIENCE
2014年
4期
302-304
,共3页
琼胶酯%抗菌活性%抗烟草花叶病毒活性
瓊膠酯%抗菌活性%抗煙草花葉病毒活性
경효지%항균활성%항연초화협병독활성
agaropectin%antibacterial activity%anti-TMV activity
以20.0 g江蓠琼脂粉为原料,经DEAE-纤维素柱层析制得琼胶酯0.46 g,产率为2.30%。抑菌圈法测定表明,琼胶酯样品对大肠杆菌和金黄色葡萄球菌无抗菌作用;采用半叶枯斑法测定,表明琼胶酯样品对烟草花叶病毒具明显的钝化作用,琼胶酯样品浓度0.05~2.0 mg·mL-1对烟草花叶病毒的抑制率为37.02%~81.51%,呈现出显著的浓度剂量效应。研究首次报道海洋红藻中的琼胶酯组分对植物病毒的抑制作用,对拓宽琼脂糖制备过程产生的副产物琼胶酯组分的用途有积极意义。
以20.0 g江蘺瓊脂粉為原料,經DEAE-纖維素柱層析製得瓊膠酯0.46 g,產率為2.30%。抑菌圈法測定錶明,瓊膠酯樣品對大腸桿菌和金黃色葡萄毬菌無抗菌作用;採用半葉枯斑法測定,錶明瓊膠酯樣品對煙草花葉病毒具明顯的鈍化作用,瓊膠酯樣品濃度0.05~2.0 mg·mL-1對煙草花葉病毒的抑製率為37.02%~81.51%,呈現齣顯著的濃度劑量效應。研究首次報道海洋紅藻中的瓊膠酯組分對植物病毒的抑製作用,對拓寬瓊脂糖製備過程產生的副產物瓊膠酯組分的用途有積極意義。
이20.0 g강리경지분위원료,경DEAE-섬유소주층석제득경효지0.46 g,산솔위2.30%。억균권법측정표명,경효지양품대대장간균화금황색포도구균무항균작용;채용반협고반법측정,표명경효지양품대연초화협병독구명현적둔화작용,경효지양품농도0.05~2.0 mg·mL-1대연초화협병독적억제솔위37.02%~81.51%,정현출현저적농도제량효응。연구수차보도해양홍조중적경효지조분대식물병독적억제작용,대탁관경지당제비과정산생적부산물경효지조분적용도유적겁의의。
The 0.46 g agaropectin was prepared from 20.0 g agar by using DEAE-cellulose. The agaropectin sample showed not any activities againstEscherichia coli and Staphylococcus aureus. However, obvious activity against tobacco mosaic virus (TMV) was observed to the agaropectin sample with a concentration-dependent manner, 37.02%—81.51% inhibition rate corresponding to 0.05—2.0 mg·mL-1agaropectin. The results presented would provide a base for further exploration of agaropectin, which was the by-product of agarose preparation.