亚热带植物科学
亞熱帶植物科學
아열대식물과학
SUBTROPICAL PLANT SCIENCE
2014年
4期
286-288
,共3页
西南桦%秋水仙素%浓度%染色体
西南樺%鞦水仙素%濃度%染色體
서남화%추수선소%농도%염색체
Betula alnoides%colchicine%concentration%chromosome
以西南桦种子无菌萌发小苗的顶芽为外植体，探讨秋水仙素的浓度、处理时间及预培养时间对西南桦染色体加倍的影响，初步建立西南桦染色体加倍的方法。结果表明，使西南桦染色体加倍的秋水仙素浓度以120 mg·L-1为宜；在秋水仙素加倍处理前，预培养10 d后再加倍15 d效果较好，此时得到的西南桦嵌合体较多。
以西南樺種子無菌萌髮小苗的頂芽為外植體，探討鞦水仙素的濃度、處理時間及預培養時間對西南樺染色體加倍的影響，初步建立西南樺染色體加倍的方法。結果錶明，使西南樺染色體加倍的鞦水仙素濃度以120 mg·L-1為宜；在鞦水仙素加倍處理前，預培養10 d後再加倍15 d效果較好，此時得到的西南樺嵌閤體較多。
이서남화충자무균맹발소묘적정아위외식체，탐토추수선소적농도、처리시간급예배양시간대서남화염색체가배적영향，초보건립서남화염색체가배적방법。결과표명，사서남화염색체가배적추수선소농도이120 mg·L-1위의；재추수선소가배처리전，예배양10 d후재가배15 d효과교호，차시득도적서남화감합체교다。
In this paper, a method for chromosome doubling ofBetula alnoideswas developed. Terminal buds from aseptic germination ofB. alnoides were used as explants, effect factors on chromosome doubling ofB. alnoides were discussed, such as concentration of colchicine, co-culture time of colchicine, preculture time. The results showed that the concentration of colchicine was better to be 120 mg·L-1 for chromosome doubling ofB. alnoides, and maximum chimaera number would be obtained in the regermination plantlet ofB. alnoides, after preculture without colchicine for 10 d and then co-culture with colchicine for 15 d.
