口腔生物医学
口腔生物醫學
구강생물의학
ORAL BIOMEDICINE
2014年
4期
177-180
,共4页
何梦颖%许小会%杨聪翀%周美玲%刘来奎
何夢穎%許小會%楊聰翀%週美玲%劉來奎
하몽영%허소회%양총충%주미령%류래규
肿瘤相关巨噬细胞%口腔鳞癌细胞%侵袭%转移%基质金属蛋白酶
腫瘤相關巨噬細胞%口腔鱗癌細胞%侵襲%轉移%基質金屬蛋白酶
종류상관거서세포%구강린암세포%침습%전이%기질금속단백매
Tumor-associated macrophages%Oral squamous cancer cells%Invasion%Metastasis%Matrix metalloproteinases
目的：探讨肿瘤相关巨噬细胞（tumor-associated macrophages，TAMs）对口腔鳞癌细胞基质金属蛋白酶（matrix metall-proteinases，MMPs）表达以及其对口腔鳞癌细胞侵袭转移的影响。方法：用佛波脂（phorbol 12-myristate 13-acetate，PMA）和巨噬细胞集落刺激因子（macrophage colony-stimulating factor，M-CSF）刺激人单核／巨噬细胞株 THP-1促其形成 TAMs，收集其上清液作为 TAMs 条件培养基培养口腔鳞癌细胞，应用实时定量 RT-PCR、Western blot 方法检测 TAMs 条件培养基作用前后口腔鳞癌细胞 MMPs 表达的差异，应用 Transwell 侵袭实验检测口腔鳞癌细胞侵袭转移能力的差异。结果：THP-1细胞在 PMA 和 M-CSF 作用后贴壁生长，分化成 TAMs。口腔癌细胞在 TAMs 条件培养基作用48 h 后，MMP-2、MMP-9、MMP-13 mRNA 的表达水平显著增高，相同条件下蛋白质的表达水平也明显增高。TAMs 条件培养基作用24 h 后的口腔鳞癌细胞侵袭转移能力显著增强。结论：TAMs 可以上调口腔鳞癌中 MMPs 的表达并促进其侵袭转移。
目的：探討腫瘤相關巨噬細胞（tumor-associated macrophages，TAMs）對口腔鱗癌細胞基質金屬蛋白酶（matrix metall-proteinases，MMPs）錶達以及其對口腔鱗癌細胞侵襲轉移的影響。方法：用彿波脂（phorbol 12-myristate 13-acetate，PMA）和巨噬細胞集落刺激因子（macrophage colony-stimulating factor，M-CSF）刺激人單覈／巨噬細胞株 THP-1促其形成 TAMs，收集其上清液作為 TAMs 條件培養基培養口腔鱗癌細胞，應用實時定量 RT-PCR、Western blot 方法檢測 TAMs 條件培養基作用前後口腔鱗癌細胞 MMPs 錶達的差異，應用 Transwell 侵襲實驗檢測口腔鱗癌細胞侵襲轉移能力的差異。結果：THP-1細胞在 PMA 和 M-CSF 作用後貼壁生長，分化成 TAMs。口腔癌細胞在 TAMs 條件培養基作用48 h 後，MMP-2、MMP-9、MMP-13 mRNA 的錶達水平顯著增高，相同條件下蛋白質的錶達水平也明顯增高。TAMs 條件培養基作用24 h 後的口腔鱗癌細胞侵襲轉移能力顯著增彊。結論：TAMs 可以上調口腔鱗癌中 MMPs 的錶達併促進其侵襲轉移。
목적：탐토종류상관거서세포（tumor-associated macrophages，TAMs）대구강린암세포기질금속단백매（matrix metall-proteinases，MMPs）표체이급기대구강린암세포침습전이적영향。방법：용불파지（phorbol 12-myristate 13-acetate，PMA）화거서세포집락자격인자（macrophage colony-stimulating factor，M-CSF）자격인단핵／거서세포주 THP-1촉기형성 TAMs，수집기상청액작위 TAMs 조건배양기배양구강린암세포，응용실시정량 RT-PCR、Western blot 방법검측 TAMs 조건배양기작용전후구강린암세포 MMPs 표체적차이，응용 Transwell 침습실험검측구강린암세포침습전이능력적차이。결과：THP-1세포재 PMA 화 M-CSF 작용후첩벽생장，분화성 TAMs。구강암세포재 TAMs 조건배양기작용48 h 후，MMP-2、MMP-9、MMP-13 mRNA 적표체수평현저증고，상동조건하단백질적표체수평야명현증고。TAMs 조건배양기작용24 h 후적구강린암세포침습전이능력현저증강。결론：TAMs 가이상조구강린암중 MMPs 적표체병촉진기침습전이。
Objective:To explore the influence of tumor-associated macrophages (TAMs)on the expression of matrix metalloprotei-nases (MMPs)and the ability of invasion and metastasis of oral squamous cancer cells.Methods:We used phorbol 12-myristate 13-acetate (PMA)and macrophage colony-stimulating factor (M-CSF)to make THP-1 monocytes differentiate into TAMs,and then we col-lected the culture media of TAMs as condition medium (CM)to stimulate oral squamous cancer cells.Real-time RT-PCR and Western blot were applied to detect the expression of MMPs before and after stimulation of TAMs.The ability of invasion and metastasis of oral squamous cells was measured by Transwell assays.Results:When treated with PMA and M-CSF,THP-1 cells became attached,and differentiated into TAMs.Compared to control group,the expression of MMP-2,MMP-9,MMP-13 mRNA in experimental group were up-regulated when treated with TAMs CM for 48h.Protein level of MMP-2,MMP-9,MMP-13 was up-regulated accordingly.And stim-ulation of TAMs CM for 24h increased invasion of oral cancer cells.Conclusions:TAMs may promote the invasion and metastasis of o-ral squamous cancer cells through enhancing production of MMPs.
