徐州工程学院学报(自然科学版)
徐州工程學院學報(自然科學版)
서주공정학원학보(자연과학판)
JOURNAL OF XUZHOU INSTITUTE OF TECHNOLOGY(NATURAL SCIENCES EDITION)
2014年
4期
47-50
,共4页
大蒜%rRNA%分类%鉴定
大蒜%rRNA%分類%鑒定
대산%rRNA%분류%감정
A llium sativ um%rRNA%phylogeny%identification
通过扩增获得18S rRNA和叶绿体16S rRNA基因序列,测序并提交GenBank ,登录号分别是JF719285和JF719286.利用大蒜和GenBank相关序列构建系统发育树,进行分子演化分析.结果表明:大蒜18S rRNA 基因与球序韭、韭菜、茖葱等葱科植物序列相似度高;叶绿体16S rRNA基因与龙舌兰科和薯蓣科的物种序列相似度高.大蒜与葱科植物在18S rRNA序列上具有较高的同源性.18S rRNA序列在植物演化方面的区分度比16S rRNA高.
通過擴增穫得18S rRNA和葉綠體16S rRNA基因序列,測序併提交GenBank ,登錄號分彆是JF719285和JF719286.利用大蒜和GenBank相關序列構建繫統髮育樹,進行分子縯化分析.結果錶明:大蒜18S rRNA 基因與毬序韭、韭菜、茖蔥等蔥科植物序列相似度高;葉綠體16S rRNA基因與龍舌蘭科和藷蕷科的物種序列相似度高.大蒜與蔥科植物在18S rRNA序列上具有較高的同源性.18S rRNA序列在植物縯化方麵的區分度比16S rRNA高.
통과확증획득18S rRNA화협록체16S rRNA기인서렬,측서병제교GenBank ,등록호분별시JF719285화JF719286.이용대산화GenBank상관서렬구건계통발육수,진행분자연화분석.결과표명:대산18S rRNA 기인여구서구、구채、각총등총과식물서렬상사도고;협록체16S rRNA기인여룡설란과화서여과적물충서렬상사도고.대산여총과식물재18S rRNA서렬상구유교고적동원성.18S rRNA서렬재식물연화방면적구분도비16S rRNA고.
In the paper ,molecular phylogeny of Allium sativum were discussded with the analysis of rRNA gene .18S rRNA gene and chloroplast 16S rRNA gene sequences were amplified .The two rRNA genes were submitted to Genbank and the accession numbers were JF719285 and JF719286 .Gene sequences of Allium sativum was analyzed with related species in GenBank .The results showed that :Allium sativum 18S rRNA gene has a high homology with many species within Alliaceae ,such as Allium thunbergii ,Allium tuberosum and Allium victorialis .Allium sativum and Alliaceae plants has a high similarity in 18S rDNA . The discrimination accusation of 18Sr RNA sequences in plant phylogeny analysis is better than that of 16S rRNA .