徐州工程学院学报（自然科学版）
徐州工程學院學報（自然科學版）
서주공정학원학보（자연과학판）
JOURNAL OF XUZHOU INSTITUTE OF TECHNOLOGY(NATURAL SCIENCES EDITION)
2014年
4期
32-38
,共7页
银杏果%提取%异黄酮%紫外分光光度法%响应面优化法
銀杏果%提取%異黃酮%紫外分光光度法%響應麵優化法
은행과%제취%이황동%자외분광광도법%향응면우화법
ginkgo fruit%extraction%isoflavone%ultraviolet spectrophotometry%response surface opti-mization experiment
以银杏果为原料，通过对索氏提取时间、固液比、乙醇体积分数、提取温度单因素试验确定最佳单因素水平并做响应面优化实验，确定了以乙醇为提取剂提取银杏果中异黄酮化合物的最佳工艺条件．然后采用紫外分光光度法测定异黄酮质量分数．结果表明，最佳提取工艺为：索氏提取时间5 h ，固液比1∶30，乙醇体积分数80％，提取温度97．45℃．在最佳条件提取后采取紫外分光光度计测定，回收率为98．15％，精密度为3．391％，银杏果异黄酮质量分数为2．255 m g／g ．
以銀杏果為原料，通過對索氏提取時間、固液比、乙醇體積分數、提取溫度單因素試驗確定最佳單因素水平併做響應麵優化實驗，確定瞭以乙醇為提取劑提取銀杏果中異黃酮化閤物的最佳工藝條件．然後採用紫外分光光度法測定異黃酮質量分數．結果錶明，最佳提取工藝為：索氏提取時間5 h ，固液比1∶30，乙醇體積分數80％，提取溫度97．45℃．在最佳條件提取後採取紫外分光光度計測定，迴收率為98．15％，精密度為3．391％，銀杏果異黃酮質量分數為2．255 m g／g ．
이은행과위원료，통과대색씨제취시간、고액비、을순체적분수、제취온도단인소시험학정최가단인소수평병주향응면우화실험，학정료이을순위제취제제취은행과중이황동화합물적최가공예조건．연후채용자외분광광도법측정이황동질량분수．결과표명，최가제취공예위：색씨제취시간5 h ，고액비1∶30，을순체적분수80％，제취온도97．45℃．재최가조건제취후채취자외분광광도계측정，회수솔위98．15％，정밀도위3．391％，은행과이황동질량분수위2．255 m g／g ．
This paper ,with ginkgo nut as raw material ,synthesized the Soxhlet extraction time ,solid‐liquid ratio ,ethanol concentration ,extraction temperature and single factor experiment to determine the op‐timal level of single factor and to do the response surface optimization experiments .Finally the optimum technological conditions for extracting the isoflavone compounds with ethanol as extracting agent had been obtained .Then the isoflavone content was also determined by the ultraviolet spectrophotometric method . The results showed that the best optimum extraction process conforms to the following conditions :soxhlet extraction time 5 h ,solid‐liquid ratio 1 ∶ 30 ,80% ethanol concentration ,extraction temperature of 97 .45DEG c .After being measured by ultraviolet spectrophotometer ,the recovery rate is 98 .15% ,the pre‐cision is 3 .391% ,ginkgo fruit isoflavone content is 2 .255 mg/g .
