CAJ | 학술논문

为了解江苏淮北小麦品种重要品质性状相关基因状况,以20份近期审定的徐麦系列和淮麦系列小麦品种以及12份小麦对照材料为试验材料,采用SDS-PAGE电泳与PCR检测相结合,检测了小麦高分子量麦谷蛋白亚基；同时采用共显性PCR标记筛查1BL/1RS易位系以及Wx基因突变型。结果显示,江苏淮北小麦品种Glu-A1位点含3种亚基,其中1亚基占75%；Glu-B1位点有4种亚基,7+8亚基占60%；Glu-D1位点有3种亚基,5+10亚基占50%；1BL/1RS易位系有14份,占全部品种的70%；所有品种均为Wx基因野生型。
위료해강소회북소맥품충중요품질성상상관기인상황,이20빈근기심정적서맥계렬화회맥계렬소맥품충이급12빈소맥대조재료위시험재료,채용SDS-PAGE전영여PCR검측상결합,검측료소맥고분자량맥곡단백아기；동시채용공현성PCR표기사사1BL/1RS역위계이급Wx기인돌변형。결과현시,강소회북소맥품충Glu-A1위점함3충아기,기중1아기점75%；Glu-B1위점유4충아기,7+8아기점60%；Glu-D1위점유3충아기,5+10아기점50%；1BL/1RS역위계유14빈,점전부품충적70%；소유품충균위Wx기인야생형。
Twenty commercia1 varieties authorized recent1y, inc1uding 9 Xumai varieties and 11 Huaimai varieties, and 12 contro1 varieties were se1ected as materia1s to identify the composition of high mo1ecu1ar weight g1utenin subunits (HMW-GS) by SDS-PAGE and PCR. The Wx mutant genes and 1BL/1RS trans1ocation 1ine were screened by PCR using co-dominant markers. Three subunit types were discovered at 1ocus Glu-A1, subunit 1 accounting for 75%. There were four subunit types at 1ocus Glu-B1, subunit 7+8 accounting for 60%. Three subunit types were identified at 1ocus Glu-D1, sub-unit 5+10 accounting for 50%. Fourteen varieties with 1BL/1RS trans1ocation were discovered, most of 1ohich (70%) came from Huaimai varieties. No Wx mutant gene was detected in a11 tested Huaibei varieties.