CAJ | 학술논문

目的：制备负载牛血清白蛋白的壳聚糖/β-甘油磷酸钠(CS/β-GP)可吸收性膜，探讨其缓释蛋白的性能。方法：利用CS/β-GP体系的温敏相转变特性，同时向其中添加蛋白制成新型生物膜。进行膜厚度及拉伸强度等力学性能测试。利用BCA蛋白浓度试剂盒（加强型）检测不同时点的蛋白浓度，绘制膜的蛋白缓释曲线。结果：利用SPSS16.0统计分析软件进行分析，负载蛋白后复合膜的理化性能均未发生明显变化（P>0.05）。不同浓度的复合膜可缓慢释放蛋白12天以上，并且随着β-GP浓度的升高，蛋白缓释总量增大。结论：新型壳聚糖温敏凝胶膜可以作为蛋白缓释的载体，是在引导骨再生领域具有应用潜力的生物膜材料。
목적：제비부재우혈청백단백적각취당/β-감유린산납(CS/β-GP)가흡수성막，탐토기완석단백적성능。방법：이용CS/β-GP체계적온민상전변특성，동시향기중첨가단백제성신형생물막。진행막후도급랍신강도등역학성능측시。이용BCA단백농도시제합（가강형）검측불동시점적단백농도，회제막적단백완석곡선。결과：이용SPSS16.0통계분석연건진행분석，부재단백후복합막적이화성능균미발생명현변화（P>0.05）。불동농도적복합막가완만석방단백12천이상，병차수착β-GP농도적승고，단백완석총량증대。결론：신형각취당온민응효막가이작위단백완석적재체，시재인도골재생영역구유응용잠력적생물막재료。
Objective:To prepare chitosan/β-Glycerophosphate salt (CS/β-GP) absorbable membranes loaded bovine serum albumin and detect the sustained-release protein properties. Methods: A newly mem-brane was synthesized added with protein,using thermal phase inversion property of the CS/β-GP sys-tem. We tested the thickness, tensile strength and the other mechanical properties of the membranes. The concentration of protein was detected at different time point by BCA protein kit(enhanced). And the protein release curve was drawn at the same time. Results:The changes of mechanical properties of the membranes were not significant, testing by SPSS16.0 statistical software(P>0.05). Membranes with dif-ferent concentration could release protein slowly more than 12 days, and the total quantity of the re-leased protein increased with the concentration of the β-GP. Conclusion:The new type CS/β-GP ther-mosensitive hydrogel membranes could be a type of protein release carrier. Also, it showed application potential in the guided bone regeneration field.