CAJ | 학술논문

目的：研究Stathmin蛋白在鼻咽癌（NPC）组织中的表达，探讨其表达与鼻咽癌临床特征之间的关联。方法①应用免疫组化检测鼻咽癌组织及正常鼻咽黏膜上皮组织（NNET ）中 Stathmin 蛋白的表达。分析Stathmin蛋白表达与鼻咽癌临床特征之间的相关性，应用Log‐Rank单因素分析Stathmin蛋白与患者预后的关系；②应用Western blot检测鼻咽癌细胞株CNE‐2、正常细胞株NP‐69细胞Stathmin蛋白的表达。结果①免疫组化发现鼻咽癌组织中Stathmin表达较NNET明显升高（P＜0．05）；②Stathmin的表达强度与鼻咽癌患者的 TNM 临床分期、T分期、N分期、M分期之间的关系有统计学意义（P＜0．05）；③单因素分析显示鼻咽癌预后与患者的临床分期、T 分期、N分期、M分期、Stathmin的表达强度之间的关系有统计学意义（P＜0．05）；④ Western blot技术检测发现鼻咽癌细胞株CNE‐2在分子量19 KD处有一特异性条带表达，正常鼻咽永生化上皮细胞株NP‐69细胞中没有表达。结论鼻咽癌组织中Stathmin表达比正常鼻咽黏膜明显上调，Stathmin在鼻咽癌组织中高表达与其 TNM临床分期、T分期、N分期、M分期显著相关，与患者的不良预后显著有关。Stathmin作为一个潜在的鼻咽癌生物标志物以及基因治疗的分子靶标值得进一步探讨。
목적：연구Stathmin단백재비인암（NPC）조직중적표체，탐토기표체여비인암림상특정지간적관련。방법①응용면역조화검측비인암조직급정상비인점막상피조직（NNET ）중 Stathmin 단백적표체。분석Stathmin단백표체여비인암림상특정지간적상관성，응용Log‐Rank단인소분석Stathmin단백여환자예후적관계；②응용Western blot검측비인암세포주CNE‐2、정상세포주NP‐69세포Stathmin단백적표체。결과①면역조화발현비인암조직중Stathmin표체교NNET명현승고（P＜0．05）；②Stathmin적표체강도여비인암환자적 TNM 림상분기、T분기、N분기、M분기지간적관계유통계학의의（P＜0．05）；③단인소분석현시비인암예후여환자적림상분기、T 분기、N분기、M분기、Stathmin적표체강도지간적관계유통계학의의（P＜0．05）；④ Western blot기술검측발현비인암세포주CNE‐2재분자량19 KD처유일특이성조대표체，정상비인영생화상피세포주NP‐69세포중몰유표체。결론비인암조직중Stathmin표체비정상비인점막명현상조，Stathmin재비인암조직중고표체여기 TNM림상분기、T분기、N분기、M분기현저상관，여환자적불량예후현저유관。Stathmin작위일개잠재적비인암생물표지물이급기인치료적분자파표치득진일보탐토。
Objective To examine the expression of Stathmin protein in nasopharyngeal carcinoma (NPC) tissuebiopsies and the association with different clinicopathologic variables .Methods The expression of Stathmin was evaluated by immunohistochemical staining of tissues from 125 cases of NPC tissue biopsies and 52 cases of normal nasopharyngeal epithelial tissues (NNET) .The connection between the expression level of Stathmin protein and clinicopathologic variables of NPC patients and the prognosis was analyzed with univariate Log‐Rank test .Be‐sides ,the expression of Stathmin protein was evaluated by Western blot in NPC cells (CNE‐2) and non‐tumor cells (NP‐69) .Results Immunohistochemistry analysis showed that the expression rate of Stathmin in NPC tissue biop‐sies was higher than in NNET tissue (P<0 .05) .The expression of Stathmin in NPC tissue biopsies was significantly correlated with TNM clinical stage ,T classification ,N classification ,and distant metastasis .Univariate analysis showed that poor prognosis of NPC was significantly associated with Stathmin expression ,TNM clinical stage ,T classification ,N classification ,and distant metastasis .In Western blot Stathmin protein showed a special band with Stathmin antibodies .Conclusions High expression of Stathmin in NPC tissues is significantly associated with dis‐tant metastasis ,TNM clinical stage ,and poor survival ,which suggests that Stathmin could serve as a good marker of prognosis and a target for new antitumor and antimetastatic therapies .