CAJ | 학술논문

目的：本实验旨在研究大鼠眶下孔注射高渗盐水阿霉素后，三叉神经功能和三叉神经节细胞病理形态的变化。方法：48只SD大鼠，随机分为A、 B两组，每组24只，每组又分四个时间点：1周组，2周组，1月组，3月组取材，每时间点6只大鼠。将A、 B组分别给予10％NaCl和1％阿霉素的混合溶液10μl眶下孔注射，生理盐水10μl眶下孔注射(B组)每组给药后于1周、2周、1月及3月时测试各组剩余大鼠的触须垫感觉功能，后处死取三叉神经节标本，制作HE染色切片，分别观察神经节细胞的形态学变化，用等距随机抽样法记录节细胞总数及异常节细胞率。结果：各组动物感觉功能测试， A、 B组同时间点有统计学差异(P<0.01)，眶下孔内神经周围注射高渗盐水阿霉素溶液后A1-A4组节细胞总数呈递减趋势(同组各时间点分别比较， P<0.01)，显著低于B组同时间点(P<0.01)，而与B组各时间点相比，无统计学差异(P>0.05)；异常节细胞率A1-A4组呈递增趋势(A组各时间点相比(P<0.01)，显著高于B组同时间点(P<0.01)。结论：大鼠眶下孔内神经周围注射高渗盐水阿霉素后，阿霉素可在高渗盐水的作用下被神经纤维大量吸收并进一步逆行运输到三叉神经节，随着时间的延续引起三叉神经节细胞的渐进性退变。
목적：본실험지재연구대서광하공주사고삼염수아매소후，삼차신경공능화삼차신경절세포병리형태적변화。방법：48지SD대서，수궤분위A、 B량조，매조24지，매조우분사개시간점：1주조，2주조，1월조，3월조취재，매시간점6지대서。장A、 B조분별급여10％NaCl화1％아매소적혼합용액10μl광하공주사，생리염수10μl광하공주사(B조)매조급약후우1주、2주、1월급3월시측시각조잉여대서적촉수점감각공능，후처사취삼차신경절표본，제작HE염색절편，분별관찰신경절세포적형태학변화，용등거수궤추양법기록절세포총수급이상절세포솔。결과：각조동물감각공능측시， A、 B조동시간점유통계학차이(P<0.01)，광하공내신경주위주사고삼염수아매소용액후A1-A4조절세포총수정체감추세(동조각시간점분별비교， P<0.01)，현저저우B조동시간점(P<0.01)，이여B조각시간점상비，무통계학차이(P>0.05)；이상절세포솔A1-A4조정체증추세(A조각시간점상비(P<0.01)，현저고우B조동시간점(P<0.01)。결론：대서광하공내신경주위주사고삼염수아매소후，아매소가재고삼염수적작용하피신경섬유대량흡수병진일보역행운수도삼차신경절，수착시간적연속인기삼차신경절세포적점진성퇴변。
Objective: The injection with compound solution of 10% NaCl and 1% adriamycin wasperformed in SD rats , tocompare the changes in function of trigeminal nerve and pathomorphology of trigeminal ganglion cells at different time points. To investigate the feasibility of treatmentof trigeminal neuralgia by perineural (infraorbital hole) injection with mixed solution of hypertonic saline and adriamycin. Methods: 48 SD rats were randomly allocated into two roups (A, B group), with 24 rats in each group. Either receiced 10ul compound solution of 10% NaCl and 1% Adriamycin (group A) or physiological saline (group B) alone were injected from the infraorbital foramen. Each group was then divided into four subgroup: 1-week subgroup, 2-week subgroup, one month subgroup, 3 month subgroup subjects, and each subgroup had six rats.At 1th week, 2th week, 1th month, 3th month after operation, the remaining rats’ sensation of the vibrissae of pad was tested, and the gasseringganglions were took out to observe the total number of ganglions cells, the ratio of abnormal ganglions cells and cells’ appearance. Results: Significant difference was found in intercomparison of rats’ sensation of the vibrissae of pad of the subgroups at the same timepoint between group A and B (P<0.01) After 10μl compound solution of 10% NaCl and 1% adriamycin injection from the infraorbital foramen, a decreasing tendency of the total number of ganglions cells was observed from subgroup 1 to 4 in group A (P<0.01), lower than that at the same timepoint in group B (P<0.01) While an increasing tendency of the ratio of abnormal ganglion cells was observed from subgroup 1 to 4 in group A (P<0.01), higher than that at the same timepoint in group B (P<0.01) No statistical difference was found among subgroups in group B. Conclusion: After 10ul compound solution of 10% NaCl and 1% adriamycin injection from the infraorbital foramen of rats, hypertonic saline maycontribute to enhancing axoplasmic transport of adriamycin, leading tonecrosis of trigeminal ganglion cells. This method could probably provide a novel and effective treatment for trigeminal neuralgia.