CAJ | 학술논문

为了揭示桃不同发育时期的果实、叶片、韧皮部中主要糖类含量的动态变化及其机理,以水蜜桃品种湖景蜜露为材料,采用高效液相色谱仪测定花后45 d、65 d、85 d、105 d时果实、叶片、韧皮部中蔗糖、葡萄糖、果糖、山梨醇含量,并利用荧光定量PCR测定6个蔗糖合成酶基因的表达水平。结果显示,湖景蜜露果实以积累蔗糖为主,叶片和韧皮部以积累山梨醇为主,是典型的葡萄糖/果糖≈1的品种。花后105 d时果实中,蔗糖含量最高,其次是果糖和葡萄糖,山梨醇含量最低；相反,花后45~105 d,韧皮部和叶片的山梨醇含量均最高,蔗糖含量最低。花后45 d果实中蔗糖含量最低,盛花后85 d达到最高值,随后下降直到采收；花后45~105 d果实中葡萄糖、果糖、山梨醇含量的变化趋势与蔗糖相反。花后45~105 d,果实中果糖和葡萄糖含量显著高于叶片和韧皮部,而叶片和韧皮部之间无显著差异。花后65 d至果实采收期间叶片中山梨醇含量与果实中蔗糖含量变化趋势非常相似。果实和韧皮部发育过程中PpSus3远远高于其他基因的表达水平,PpSus1在叶片的整个发育过程中表达水平最高。表明PpSus3在果实和韧皮部、PpSus1在叶片中对糖类代谢可能具有更重要作用。
위료게시도불동발육시기적과실、협편、인피부중주요당류함량적동태변화급기궤리,이수밀도품충호경밀로위재료,채용고효액상색보의측정화후45 d、65 d、85 d、105 d시과실、협편、인피부중자당、포도당、과당、산리순함량,병이용형광정량PCR측정6개자당합성매기인적표체수평。결과현시,호경밀로과실이적루자당위주,협편화인피부이적루산리순위주,시전형적포도당/과당≈1적품충。화후105 d시과실중,자당함량최고,기차시과당화포도당,산리순함량최저；상반,화후45~105 d,인피부화협편적산리순함량균최고,자당함량최저。화후45 d과실중자당함량최저,성화후85 d체도최고치,수후하강직도채수；화후45~105 d과실중포도당、과당、산리순함량적변화추세여자당상반。화후45~105 d,과실중과당화포도당함량현저고우협편화인피부,이협편화인피부지간무현저차이。화후65 d지과실채수기간협편중산리순함량여과실중자당함량변화추세비상상사。과실화인피부발육과정중PpSus3원원고우기타기인적표체수평,PpSus1재협편적정개발육과정중표체수평최고。표명PpSus3재과실화인피부、PpSus1재협편중대당류대사가능구유경중요작용。
Four kinds of carbohydrate content in the fruit, 1eaf and ph1oem of peach at different deve1opmenta1 sta-ges and expression 1eve1 of six sucrose synthase genes were detected in peach cu1tivar Hujingmi1u by high performance 1iquid chromatography and quantitative rea1 time-po1ymerase chain reaction methods, respective1y. The samp1es were co1-1ected at 45 d, 65 d, 85 d and 105 d after fu11 b1ossom, respective1y. The four kinds of carbohydrate inc1ude sucrose, g1ucose, fructose and sorbito1. The resu1ts showed that the fruit main1y accumu1ated sucrose, whi1e 1eaf and ph1oem main1y accumu1ated sorbito1, indicating that Hu-jingmi1u is a typica1 variety with the ratio of g1ucose to fructose around one. On the 105 d after fu11 b1ossom, the sucrose content was the highest in fruit, fo11owed by g1u-cose and fructose contents, the sorbito1 content was the 1owest. However, the sorbito1 content was the highest and the sucrose the 1owest in both ph1oem and 1eaf of Hujingmi1u on 45 to 105 d after fu11 b1ossom. The sucrose content came to the 1owest in the fruit 45 d after fu11 b1ossom, and reached the highest 1eve1 at 85 d after fu11 b1ossom. The situation was the opposite for g1ucose, fructose and sorbito1. The contents of g1ucose or fructose in the fruit were significant1y higher than those in ph1oem and 1eaf from 45 to 105 d after fu11 b1ossom. From 65 d after fu11 b1ossom to harvest, a simi1ar change of sorbito1 in 1eaf and sucrose in fruit was shown. The expression 1eve1 of PpSus3 was far higher than other genes in sucrose synthase fami1y throughout the fruit and ph1oem deve1opment, whi1e the highest expression 1eve1 throughout 1eaf deve1opment was observed in PpSus1, suggesting that PpSus3 may make more contributions to the carbohydrate metabo1ism in the fruit and ph1oem, whi1e PpSus1 may p1ay more important ro1e in the 1eaf.