CAJ | 학술논문

为揭示解草啶浸种减轻丙草胺对水稻药害的机制，采用生物化学方法研究解草啶浸种对水稻的谷胱甘肽巯基转移酶( GST)活性、琢-淀粉酶活性、叶绿素含量和蛋白质含量的影响.结果显示：单独施用30 mg/L解草啶浸种能提高水稻GST活性，但对琢-淀粉酶活性、叶绿素含量和蛋白质含量没有影响.单独施用不同剂量(56.25 g/hm2、112.50 g/hm2、225.00 g/hm2)丙草胺后，低剂量丙草胺对GST活性有促进作用，高剂量丙草胺对GST活性有抑制作用；不同剂量丙草胺对琢-淀粉酶活性、叶绿素含量和蛋白质含量均有抑制作用.通过30 mg/L解草啶浸种再施用不同剂量丙草胺，解草啶均能减轻丙草胺对水稻GST活性、琢-淀粉酶活性、叶绿素含量和蛋白质含量的抑制作用.
위게시해초정침충감경병초알대수도약해적궤제，채용생물화학방법연구해초정침충대수도적곡광감태구기전이매( GST)활성、탁-정분매활성、협록소함량화단백질함량적영향.결과현시：단독시용30 mg/L해초정침충능제고수도GST활성，단대탁-정분매활성、협록소함량화단백질함량몰유영향.단독시용불동제량(56.25 g/hm2、112.50 g/hm2、225.00 g/hm2)병초알후，저제량병초알대GST활성유촉진작용，고제량병초알대GST활성유억제작용；불동제량병초알대탁-정분매활성、협록소함량화단백질함량균유억제작용.통과30 mg/L해초정침충재시용불동제량병초알，해초정균능감경병초알대수도GST활성、탁-정분매활성、협록소함량화단백질함량적억제작용.
To revea1 the mechanism of fenc1orim reducing the phytotoxicity of preti1ach1or to rice by seed soaking, biochemica1 tests were conducted to study the effects of fenc1orim soaking on g1utathione S-transferase( GST) activity, α-amy1ase activity, and contents of ch1orophy11 and protein of rice. The resu1ts showed that GST activity was enhanced by 30 mg/L fenc1orim, however,α-amy1ase, contents of ch1orophy11 and protein were not affected. Low dose of preti1ach1or cou1d increase GST activity whi1e high dose showed inhibition. A11 doses of preti1ach1or exhibited negative effect onα-amy1ase ac-tivity, contents of ch1orophy11 and protein. The negative effect on GST activity,α-amy1ase activity, contents of ch1orophy11 and protein of rice cou1d be reduced by seed soaking with 30 mg/L fenc1orim.