江苏农业学报
江囌農業學報
강소농업학보
JIANGSU JOURNAL OF AGRICULTURAL SCIENCES
2014年
6期
1304-1308
,共5页
乔俊卿%夏彦飞%刘邮洲%梁雪杰%杜艳
喬俊卿%夏彥飛%劉郵洲%樑雪傑%杜豔
교준경%하언비%류유주%량설걸%두염
枯草芽孢杆菌PTS-394%根系活力%根际微生物群体%土壤酶活性
枯草芽孢桿菌PTS-394%根繫活力%根際微生物群體%土壤酶活性
고초아포간균PTS-394%근계활력%근제미생물군체%토양매활성
Bacillus subtilis PTS-394%root activity%microbia1 community in rhizosphere%soi1 enzyme activity
为了明确枯草芽孢杆菌( Bacillus subtilis) PTS-394对番茄根系及根围微生态的影响,本研究采用生理生化方法测定了番茄根系活力和根围土壤酶活性,并测定了番茄根冠比及根系周围可培养微生物的数量.结果表明,枯草芽孢杆菌PTS-394能够显著促进番茄根系生长,促进生长率达25%;同时PTS-394还能够提高根系活力,在PTS-394处理后第12 h、24 h,根系活力均比对照显著增高,增高比例分别为17.0%和25.6%,随后根系活力和对照基本持平并保持一致.枯草芽孢杆菌PTS-394的施用对番茄根围土壤中的放线菌具有显著的促进作用,对真菌具有轻微的抑制作用,而对细菌则无明显作用.此外,PTS-394能够促进番茄根围土壤脲酶和土壤蔗糖酶的活性,但其对2种酶的作用均有时效性,其影响会随着时间的变化而逐渐减弱.
為瞭明確枯草芽孢桿菌( Bacillus subtilis) PTS-394對番茄根繫及根圍微生態的影響,本研究採用生理生化方法測定瞭番茄根繫活力和根圍土壤酶活性,併測定瞭番茄根冠比及根繫週圍可培養微生物的數量.結果錶明,枯草芽孢桿菌PTS-394能夠顯著促進番茄根繫生長,促進生長率達25%;同時PTS-394還能夠提高根繫活力,在PTS-394處理後第12 h、24 h,根繫活力均比對照顯著增高,增高比例分彆為17.0%和25.6%,隨後根繫活力和對照基本持平併保持一緻.枯草芽孢桿菌PTS-394的施用對番茄根圍土壤中的放線菌具有顯著的促進作用,對真菌具有輕微的抑製作用,而對細菌則無明顯作用.此外,PTS-394能夠促進番茄根圍土壤脲酶和土壤蔗糖酶的活性,但其對2種酶的作用均有時效性,其影響會隨著時間的變化而逐漸減弱.
위료명학고초아포간균( Bacillus subtilis) PTS-394대번가근계급근위미생태적영향,본연구채용생리생화방법측정료번가근계활력화근위토양매활성,병측정료번가근관비급근계주위가배양미생물적수량.결과표명,고초아포간균PTS-394능구현저촉진번가근계생장,촉진생장솔체25%;동시PTS-394환능구제고근계활력,재PTS-394처리후제12 h、24 h,근계활력균비대조현저증고,증고비례분별위17.0%화25.6%,수후근계활력화대조기본지평병보지일치.고초아포간균PTS-394적시용대번가근위토양중적방선균구유현저적촉진작용,대진균구유경미적억제작용,이대세균칙무명현작용.차외,PTS-394능구촉진번가근위토양뇨매화토양자당매적활성,단기대2충매적작용균유시효성,기영향회수착시간적변화이축점감약.
To figure out the effect of Bacillus subtilis PTS-394 on the microbia1 community in rhizosphere, the tomato root 1ength, root activity, the amount of microbia1 of rhizosphere and soi1 enzyme activities were measured. The resu1ts showed that B. subtilis PTS-394 improved the growth of tomato root by 25% and root vigor by 17. 0% and 25. 6% at 12-h and 24-h posttreatment, respective1y. After the app1ication of PTS-394, the amount of rhizosphere soi1 actinomycetes was higher than contro1, the amount of fungi was suppressed s1ight1y, however the bacteria was not inf1uenced. PTS-394 im-proved the activities of soi1 urease and sucrase in tomato rhizosphere. The improvement was time-specific and was getting weaker over time.