CAJ | 학술논문

为了探讨盐胁迫对香蕉水通道蛋白质基因( MaAQP1)转基因拟南芥幼苗的生长及抗逆性生理指标的影响，以MaAQP1转基因拟南芥为研究对象，研究不同盐( NaC1)浓度(0 mmo1/L、50 mmo1/L、100 mmo1/L、150 mmo1/L)处理下转基因拟南芥的表型及生理指标变化。结果显示：(1)经过盐胁迫后，野生型和转基因株系钾离子( K+)浓度降低，钠离子( Na+)浓度升高，而转基因株系K+和Na+浓度均明显低于野生型株系，同时，转基因株系具有较高的K+/Na+；(2)随着盐胁迫浓度的增加，野生型和转基因株系中电导率、丙二醛含量、脯氨酸含量及过氧化氢酶活性均逐渐升高，当盐胁迫浓度达到150 mmo1/L时4个指标均达到高峰，其中，转基因株系中的电导率、脯氨酸含量及过氧化氢酶活性均比野生型的高，而丙二醛含量比野生型的低；(3)随着盐胁迫浓度的增加，野生型株系幼苗的根长缩短程度大于转基因株系，同时，转基因株系的根毛要远多于野生型。表明MaAQP1能够提高转基因拟南芥的耐盐性。
위료탐토염협박대향초수통도단백질기인( MaAQP1)전기인의남개유묘적생장급항역성생리지표적영향，이MaAQP1전기인의남개위연구대상，연구불동염( NaC1)농도(0 mmo1/L、50 mmo1/L、100 mmo1/L、150 mmo1/L)처리하전기인의남개적표형급생리지표변화。결과현시：(1)경과염협박후，야생형화전기인주계갑리자( K+)농도강저，납리자( Na+)농도승고，이전기인주계K+화Na+농도균명현저우야생형주계，동시，전기인주계구유교고적K+/Na+；(2)수착염협박농도적증가，야생형화전기인주계중전도솔、병이철함량、포안산함량급과양화경매활성균축점승고，당염협박농도체도150 mmo1/L시4개지표균체도고봉，기중，전기인주계중적전도솔、포안산함량급과양화경매활성균비야생형적고，이병이철함량비야생형적저；(3)수착염협박농도적증가，야생형주계유묘적근장축단정도대우전기인주계，동시，전기인주계적근모요원다우야생형。표명MaAQP1능구제고전기인의남개적내염성。
The changes of seed1ing growth and physio1ogica1 indexes of MaAQP1-transgenic Arabidopsis thaliana un-der different concentrations of NaC1 stress at 0 mmo1/L, 50 mmo1/L, 100 mmo1/L, and 150 mmo1/L were detected. Under NaC1 stress, K+ concentration in wi1d-type and transgenic p1ants were reduced, whi1e Na+ con-centration were increased. K+and Na+ concentrations in transgenic p1ants were significant1y 1ower than those in the wi1d-type p1ants, however, K+/Na+ ratio was higher in transgenic 1ines. With the increase of sa1t concentration, the ion 1eakage, ma1ondia1dehyde content, pro1ine content and cat1yse( CAT) activity in both transgenic 1ines and wi1d type were increased gradua11y, and reached the peak va1ues at sa1t concentration of 150 mmo1/L. The ion 1eakage, pro1ine content and CAT activity in the transgenic 1ines were higher than those in the wi1d-type p1ants, whi1e the MDA content was 1ower. With the increase of sa1t concentration, the root 1ength in wi1d-type 1ines seed1ing was shortened more than that in transgenic 1ines whose root hairs were far more than wi1d type’ s. These resu1ts suggested that MaAQP1 cou1d improve sa1t to1erance of transgenic A. thaliana.