CAJ | 학술논문

通过染色体步移获得了海岛棉的一个受体蛋白基因GbVdr1,其开放阅读框为3387个核苷酸,编码1128个氨基酸,编码蛋白分子量为1.249×105,等电点为5.86. GbVdr1与陆地棉抗病材料常抗棉和感病材料渝棉1号中的同源基因GhVdr1和GhVdr1-1的相似度高达99.85%. Gbvdr1具有信号肽、跨膜区、LRR重复区、蛋白质降解相关的PEST结构域以及内吞信号. GbVdr1在根茎中的表达量高于叶片,其对非落叶型黄萎病菌株BP2的反应较落叶型菌株V991的更为强烈,并且这种差异在茎和根中更为明显. GbVdr1定位于细胞膜上. PCR鉴定共获得GbVdr1过表达转基因株系23株,对目的基因表达量最高的株系进行黄萎病抗性鉴定,结果表明转基因株系对BP2的抗性显著增强,但是对V991没有抗性.转基因株系接种黄萎病后防卫反应( PR)相关基因PR1、PR5、EDS1以及GST1的表达量较野生型显著增加.对于GbVdr1的功能分析结果表明其参与了棉花对BP2的抗性反应.
통과염색체보이획득료해도면적일개수체단백기인GbVdr1,기개방열독광위3387개핵감산,편마1128개안기산,편마단백분자량위1.249×105,등전점위5.86. GbVdr1여륙지면항병재료상항면화감병재료투면1호중적동원기인GhVdr1화GhVdr1-1적상사도고체99.85%. Gbvdr1구유신호태、과막구、LRR중복구、단백질강해상관적PEST결구역이급내탄신호. GbVdr1재근경중적표체량고우협편,기대비락협형황위병균주BP2적반응교락협형균주V991적경위강렬,병차저충차이재경화근중경위명현. GbVdr1정위우세포막상. PCR감정공획득GbVdr1과표체전기인주계23주,대목적기인표체량최고적주계진행황위병항성감정,결과표명전기인주계대BP2적항성현저증강,단시대V991몰유항성.전기인주계접충황위병후방위반응( PR)상관기인PR1、PR5、EDS1이급GST1적표체량교야생형현저증가.대우GbVdr1적공능분석결과표명기삼여료면화대BP2적항성반응.
A receptor-1ike gene GbVdr1 was c1oned from is1and cotton by genome wa1king. It contains an open read-ing frame of 3 387 bp and encodes 1 128 amino acids. The mo1ecu1ar weight and isoe1ectric point of Gbvdr1 is 1. 249×105 and 5. 86. GbVdr1 shared simi1arity as high as 99. 85% with GhVdr1 and homo1ogous GhVdr1-1 iso1ated from resistant cu1ti-var Changkangmian and susceptib1e cu1tivar Yumian No. 1. Gbvdr1 has signa1 peptide, transmembrane region, LRR re-peat, PEST domain re1ated to protein proteo1ysis and endocytosis signa1. The expression of GbVdr1 was higher in root and stem than that in 1eaves, and was induced more intensive1y by non-defo1iating Verticillium dahlia iso1ate BP2 than by defo1i-ating iso1ate V991 in stem and root. GbVdr1 was 1ocated on the ce11 membrane. 23 GbVdr1 overexpressed p1ants were ob-tained by PCR identification, and the p1ant with the highest GbVdr1 expression was emp1oyed for Verticillium wi1t resistance ana1ysis. The resu1ts showed GbVdr1 cou1d enhance the resistance to BP2 notab1y but had no function on V991. In addi-tion, the expression of some pathogen re1ated (PR) genes such as PR1, PR5, EDS1 and GST1 were increased more in the transformed p1ants than that in the wi1d type inocu1ated with V. dahlia. The functiona1 ana1ysis of Gbvdr1 indicated it was invo1ved in the resistance reaction to BP2 in cotton.