CAJ | 학술논문

本研究对一个叶夹角变小、叶片短而直立的突变体ser( Short and erect)进行了植物激素敏感性试验和基因定位.激素试验结果表明,ser对赤霉素( Gibbere11ic acid, GA)敏感而对油菜素内酯( Brassino1ide, BR)敏感性降低.遗传分析结果表明,ser的直立叶性状受1对隐性单基因ser控制.在ser×08CR578 F2群体中,利用分子标记将ser基因定位在水稻第5染色体长臂上的标记RM3437与RM5454之间,ser基因距离2个标记的遗传距离分别为5.3 cM和1.5 cM；在ser×L604 F2群体中,ser基因定位于第5染色体上标记RM18504与RM1237之间,距离2个标记的遗传距离分别为1.5 cM和2.1 cM.在2个群体中,ser基因均与标记RM18532共分离.本研究为进一步精细定位与克隆ser基因奠定了基础,也为解析水稻叶夹角变化机制及选育高产理想株型水稻提供了理论支撑和基因资源.
본연구대일개협협각변소、협편단이직립적돌변체ser( Short and erect)진행료식물격소민감성시험화기인정위.격소시험결과표명,ser대적매소( Gibbere11ic acid, GA)민감이대유채소내지( Brassino1ide, BR)민감성강저.유전분석결과표명,ser적직립협성상수1대은성단기인ser공제.재ser×08CR578 F2군체중,이용분자표기장ser기인정위재수도제5염색체장비상적표기RM3437여RM5454지간,ser기인거리2개표기적유전거리분별위5.3 cM화1.5 cM；재ser×L604 F2군체중,ser기인정위우제5염색체상표기RM18504여RM1237지간,거리2개표기적유전거리분별위1.5 cM화2.1 cM.재2개군체중,ser기인균여표기RM18532공분리.본연구위진일보정세정위여극륭ser기인전정료기출,야위해석수도협협각변화궤제급선육고산이상주형수도제공료이론지탱화기인자원.
A short and erect rice (ser) mutant was used for phytohormone sensitivity ana1ysis and genetic mapping. Phy-tohormone treatment showed that the mutant was sensitive to gibbere11ic acid ( GA) and insensitive to brassino1ide ( BR) . Genetic ana1ysis revea1ed that the erect 1eaf was governed by a recessive nuc1eus gene, ser. The ser gene was first1y identified with 500 pairs of simp1e sequence repeat (SSR) markers covering the who1e genome by BSA with F2 popu1ations derived from F1s of ser× 08CR578 and ser×L604. In F2 popu1ation of ser×08CR578, ser gene was 1ocated on the 1ong arm of chromosome 5 between RM3437 and RM5454, with genetic distances of 5. 3 cM and 1. 5 cM respective1y, and co-segregated with RM18532. In F2 popu-1ation of ser×L604, ser gene was 1ocated on the 1ong arm of chromosome 5 between RM18504 and RM1237, with genetic distances of 1. 5 cM and 2. 1 cM, respective1y, and co-segregated with RM18532 as we11. The study 1aid the foundation for further fine-mapping and c1oning of the gene, and wou1d contribute to the defining of regu1ation mechanism of 1eaf ang1e and supp1y a genetic resource for breeding ideotype rice.