CAJ | 학술논문

目的：建立绿色荧光蛋白（ GFP）裸鼠结肠癌肿瘤模型，并与普通裸鼠结肠癌肿瘤模型进行比较，观察其在肿瘤形态学、生物学特性及病理学之间的差异。方法将处于对数生长期的KM12SM人结肠癌细胞分别接种于10只GFP裸鼠和10只裸鼠的右侧腋窝皮下，观察成瘤时间，肿瘤体积及病理形态等情况。结果 GFP裸鼠平均成瘤时间约为4～5 d，裸鼠约为6～8 d；接种第20天，裸鼠组测得体积约为（3463.43±341.39）mm3，GFP裸鼠组肿瘤体积约为（4923.46±521.72） mm3，存在显著差异。镜下见肿瘤细胞生长活跃，大小较为均匀，部分区域可见腺腔形成。结论经比较研究，GFP裸鼠在结肠癌肿瘤模型的成瘤时间，肿瘤质量等方面较裸鼠具有一定的优势，可为结肠癌转移的机制探讨和诊断治疗研究提供更为理想的动物模型。
목적：건립록색형광단백（ GFP）라서결장암종류모형，병여보통라서결장암종류모형진행비교，관찰기재종류형태학、생물학특성급병이학지간적차이。방법장처우대수생장기적KM12SM인결장암세포분별접충우10지GFP라서화10지라서적우측액와피하，관찰성류시간，종류체적급병리형태등정황。결과 GFP라서평균성류시간약위4～5 d，라서약위6～8 d；접충제20천，라서조측득체적약위（3463.43±341.39）mm3，GFP라서조종류체적약위（4923.46±521.72） mm3，존재현저차이。경하견종류세포생장활약，대소교위균균，부분구역가견선강형성。결론경비교연구，GFP라서재결장암종류모형적성류시간，종류질량등방면교라서구유일정적우세，가위결장암전이적궤제탐토화진단치료연구제공경위이상적동물모형。
Objective To establish ectopic colon tumor model in GFP nude, make a comparative study of the differences between tumor morphology, biological characteristics and pathology with ectopic colon tumor model in BALB/c nude.Methods Use KM12SM colon cancer cells of the logarithmic growth phase seeded in right axillary subcutaneous on the GFP nude and BALB/c nude.Observed time of tumor formation, tumor volume, pathological conditions and other. Results The average time of tumor formation in GFP nude was 4~5 d, BALB/c nude was 6~8 d;The first 20 days after inoculation, tumor volume of BALB/c nude group was about(3463.43 ±341.39)mm3, GFP nude was about(4923.46 ± 521.72)mm3.There are significant differences.Tumor cells growth activity, have relatively uniform size, some areas visible glandular formation on the microscope.Conclusion By comparison study, the GFP nude have certain advantages on the time of tumor formation, quality and other aspects in colon tumor model than the BALB/c nude.Provide more desirable animal model for investigating the mechanism of colon cancer lymph node metastasis, diagnosis and treatment.