CAJ | 학술논문

目的：将黑线仓鼠及其白化突变系肌张力异常蛋白变体X1基因编码区进行序列比对分析，试揭示黑线仓鼠及其白化突变系肌张力异常蛋白变体X1基因编码区之间是否存在变异。方法根据小鼠与大鼠的同种型肌张力异常蛋白变体X1段基因设计6对引物，采用RT-PCR的方法，从黑线仓鼠及其白化系皮肤中扩增得到cDNA基因，并对其二者进行克隆测序。结果将由RT-PCR方法获得的基因序列进行比对后可知，二者编码区共有17处差异，氨基酸差异共24处，但无关键核酸蛋白出现变异现象。结论黑线仓鼠白化突变系白化性状产生，并不是由肌张力异常蛋白变体X1的这段基因编码区突变造成的，其白化性状产生的机制尚需要进一步的研究。
목적：장흑선창서급기백화돌변계기장력이상단백변체X1기인편마구진행서렬비대분석，시게시흑선창서급기백화돌변계기장력이상단백변체X1기인편마구지간시부존재변이。방법근거소서여대서적동충형기장력이상단백변체X1단기인설계6대인물，채용RT-PCR적방법，종흑선창서급기백화계피부중확증득도cDNA기인，병대기이자진행극륭측서。결과장유RT-PCR방법획득적기인서렬진행비대후가지，이자편마구공유17처차이，안기산차이공24처，단무관건핵산단백출현변이현상。결론흑선창서백화돌변계백화성상산생，병불시유기장력이상단백변체X1적저단기인편마구돌변조성적，기백화성상산생적궤제상수요진일보적연구。
Objective To clone and sequence the dystonin variant X1 gene of Cricetulusbarabensis and the albino mutant Cricetulusbarabensis so as to find out the difference of encoding arear of the muscular ribosome between Cricetulusbarabensis and the albino mutant Cricetulusbarabensis.Methods According to the same type of abnormal muscle tone protein of the mice and rats, we designed 6 pairs of primers, and got their cDNA genes from skins of the Cricetulusbarabensis and the albino mutant Cricetulusbarabensis by RT-PCR amplification, then cloned and sequenced. Results Sequence alignment showed 17 variances in coding areas, and 24 in amino acid, but no in key nucleic acid and protein.Conclusion The variances in coding areas will not lead to the albino, and its mechanism requires further investigation.