CAJ | 학술논문

目的：为了找到并克隆出影响长爪沙鼠不同willis血管类型表达的基因，建立包含各种血管类型的全基因组文库。方法选取清洁级长爪沙鼠96只，解剖后剥离不同willis血管类型，分别提取DNA后混合。选用Copy Control Fosmid Library Kit和pCC1FOS载体构建文库，并对文库进拷贝数、重组片段长度及重组率进行鉴定。结果成功建立了长爪沙鼠不同willis血管类型的全基因组文库，文库容量为1700个拷贝数，插入重组片段长度约为36 kb，重组率为93％。结论建立了不同willis血管类型的长爪沙鼠全基因组文库，为后续基因克隆及筛选等研究打下良好基础。
목적：위료조도병극륭출영향장조사서불동willis혈관류형표체적기인，건립포함각충혈관류형적전기인조문고。방법선취청길급장조사서96지，해부후박리불동willis혈관류형，분별제취DNA후혼합。선용Copy Control Fosmid Library Kit화pCC1FOS재체구건문고，병대문고진고패수、중조편단장도급중조솔진행감정。결과성공건립료장조사서불동willis혈관류형적전기인조문고，문고용량위1700개고패수，삽입중조편단장도약위36 kb，중조솔위93％。결론건립료불동willis혈관류형적장조사서전기인조문고，위후속기인극륭급사선등연구타하량호기출。
Objective To find out and clone the genes that can influence different willis circle in Meriones unguiculatus, the genomic library has been made.Methods 96 clean Meriones unguiculatus were dissected according to observe the differences of willis circle, and the mixed DNA was extracted from different blood vessels.The genomic library construction has been done by using pCC1FOS vector, following by CopyControl Fosmid Lib manual.The copy numbers, recombined segment size and recombination fraction of the library were measured.Results Genomic library of different Willis circle in Meriones unguiculatus was successfully made.The capacity of the library was 1700 copies.The segment size was 36kb, and the recombination fraction was 93%.Conclusion The library was the good beginning for the following steps which were gene cloning and genescreen.