CAJ | 학술논문

目的：鉴定猴B病毒囊膜蛋白gD的抗原表位。方法利用生物信息学分析猴B病毒囊膜蛋白gD的二级结构、亲水性、表面可及性、抗原性指数以及柔韧性，得到可能的表位肽段；再利用合成肽与B病毒阳性血清进行ELISA验证，评价表位肽段的特异性和敏感性。结果在gD蛋白上预测得到7个表位肽段；ELISA测定结果显示，4个肽段（序列分别为46 LPPLEQKTD54、106 RGAPEATRSDA116、291 PELAPEERGTSRTPGD306和361 AVYLVRRRGR370）可与B病毒阳性血清发生免疫学反应，敏感性在40％～70％之间。结论 B病毒gD蛋白上至少存在4个线性化表位。
목적：감정후B병독낭막단백gD적항원표위。방법이용생물신식학분석후B병독낭막단백gD적이급결구、친수성、표면가급성、항원성지수이급유인성，득도가능적표위태단；재이용합성태여B병독양성혈청진행ELISA험증，평개표위태단적특이성화민감성。결과재gD단백상예측득도7개표위태단；ELISA측정결과현시，4개태단（서렬분별위46 LPPLEQKTD54、106 RGAPEATRSDA116、291 PELAPEERGTSRTPGD306화361 AVYLVRRRGR370）가여B병독양성혈청발생면역학반응，민감성재40％～70％지간。결론 B병독gD단백상지소존재4개선성화표위。
Objective To Identify B-cell epitopes on monkey B virus envelope protein gD.Methods Base on bioinformatics software, secondary structure, hydrophilicity, surface accessibility, antigenic index and flexibility of monkey B virus envelope protein gD was analysed and some potential peptide epitopes were forecasted.Then, the interactions between synthetic peptides and BV positive sera were detected by Enzyme-linked immunosorbent assay ( ELISA) .At last, The sensitivity and specificity of synthetic peptides was evaluated used 20 samples of Standard sera by ELISA.Result Seven epitopes were forecasted by Bioinformatics analysis. Four synthetic peptides, sequence as 46 LPPLEQKTD54 , 106 RGAPEATRSDA116 , 291 PELAPEERGTSRTPGD306 and 361 AVYLVRRRGR370 could be reacted with positive sera pool.The sensitivity of 4 synthetic peptides changed form 40% to 70% and the specificity were 100% for 4 synthetic peptides. Conclusion There are at least four linear epitope on B virus gD protein.