CAJ | 학술논문

目的：建立兔实验性动脉粥样硬化和心肌梗死双模型，观察回心草提取液对兔动脉粥样硬化斑块和缺血心肌血管新生的影响。方法选择30只家兔，随机分为3组，普通饲料对照组（对照组），高脂饲料组（高脂组），回心草干预组（干预组），每组各10只，共喂养9周。第9周末，采用开胸结扎兔冠状动脉左前降支（LAD）的方法，建立兔急性心肌梗死模型。实验终点时，测定血液生化指标，对血管及心脏组织行病理学检查，免疫组化染色测定心肌组织分化抗原簇34（CD34）及血管内皮细胞生长因子受体2（VEGFR2）阳性反应强度。结果3组比较，高脂组的肌酸激酶同工酶（CK-MB）、肌钙蛋白I（TnI）、总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）明显升高，差异具有统计学意义（P＜0.01）；干预组与高脂组比较，TC、TG、CK-MB、TnI明显下降（P＜0.01）， LDL-C下降（P＜0.05）。高脂组与对照组病理评分比较，差异有统计学意义（P＜0.01）；干预组的病理评分较对照组高，较高脂组低（P＜0.05）。高脂组心肌组织CD34和VEGFR2阳性组织相对灰度值均明显高于对照组（P＜0.01）；干预组VEGFR2及CD34表达水平较对照组高、较高脂组低（P＜0.05）。结论高脂饲料喂养和开胸结扎兔冠状动脉LAD的方法可成功建立家兔动脉粥样硬化和急性心肌梗死双模型。回心草提取液减轻了动脉粥样硬化和心肌缺血坏死，抑制了心肌组织CD34和VEGFR2的表达。
목적：건립토실험성동맥죽양경화화심기경사쌍모형，관찰회심초제취액대토동맥죽양경화반괴화결혈심기혈관신생적영향。방법선택30지가토，수궤분위3조，보통사료대조조（대조조），고지사료조（고지조），회심초간예조（간예조），매조각10지，공위양9주。제9주말，채용개흉결찰토관상동맥좌전강지（LAD）적방법，건립토급성심기경사모형。실험종점시，측정혈액생화지표，대혈관급심장조직행병이학검사，면역조화염색측정심기조직분화항원족34（CD34）급혈관내피세포생장인자수체2（VEGFR2）양성반응강도。결과3조비교，고지조적기산격매동공매（CK-MB）、기개단백I（TnI）、총담고순（TC）、감유삼지（TG）、저밀도지단백담고순（LDL-C）명현승고，차이구유통계학의의（P＜0.01）；간예조여고지조비교，TC、TG、CK-MB、TnI명현하강（P＜0.01）， LDL-C하강（P＜0.05）。고지조여대조조병리평분비교，차이유통계학의의（P＜0.01）；간예조적병리평분교대조조고，교고지조저（P＜0.05）。고지조심기조직CD34화VEGFR2양성조직상대회도치균명현고우대조조（P＜0.01）；간예조VEGFR2급CD34표체수평교대조조고、교고지조저（P＜0.05）。결론고지사료위양화개흉결찰토관상동맥LAD적방법가성공건립가토동맥죽양경화화급성심기경사쌍모형。회심초제취액감경료동맥죽양경화화심기결혈배사，억제료심기조직CD34화VEGFR2적표체。
ObjectiveTo establish a rabbit model of atherosclerosis (AS) and acute myocardial infarction (AMI), and then to determine the effect of the extracts ofRhodobryum roseum Limpr., a Chinese herbal medicine on the rabbit AS and ischemic myocardial angiogenesis.Methods A total of 30 white rabbits were randomly and equally divided into 3 groups, that is, normal control group (fed with the full diet), high-fat feed group andR. roseum intervention group (fed with high fat diet and intra-gastric injection of the extract). At the end of the 9 weeks’ feeding, AMI was inflicted by opening chest and ligating the left anterior descending artery (LAD). At the end of experiment, the blood biochemical indicators were determined, and blood vessels and heart tissue were collected for pathological observation. Immunohistochemical staining was used to detect the expression of the cluster of differentiation 34 (CD34) and vascular endothelial growth factor receptor 2 (VEGFR2) in the myocardium.Results Compared with the control group, the high-fat group and the intervention group had significantly increased creatine kinase isoenzyme (CK-MB), troponin I (TnI), total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C) (P<0.01). The serum levels of TC, TG, CK-MB and TnI were very significantly decreased (P<0.01), and that of LDL-C was obviously reduced (P<0.05) in the intervention group than in the high-fat group. The pathological grade was very significantly higher in the high-fat group thanin the control group (P<0.01), and the grade of the intervention group was between those of the former groups (P<0.05). CD34 and VEGFR2 were significantly expressed with stronger positive intensity in the high-fat group than in the control group (P<0.01). Their expression levels in the intervention group were obviously lower than those of high fat group but higher than those of the control group (P<0.05).Conclusion High fat feeding for 9 weeks and ligating LAD artery can successfully establish a rabbit model of AS and AMI. The extract ofR. roseum attenuates AS and myocardial ischemic necrosis and inhibits the expression of CD34 and VEGFR2 in the myocardium.