中国人兽共患病学报
中國人獸共患病學報
중국인수공환병학보
CHINESE JOURNAL OF ZOONOSES
2014年
12期
1234-1238
,共5页
张涛%宁文艳%吴建华%刘京利%乔富贵%李贵昌%鲁亮%刘起勇%李丽%赵建华
張濤%寧文豔%吳建華%劉京利%喬富貴%李貴昌%魯亮%劉起勇%李麗%趙建華
장도%저문염%오건화%류경리%교부귀%리귀창%로량%류기용%리려%조건화
DNA条形码%自然疫源地%啮齿动物%隐含种%宁夏
DNA條形碼%自然疫源地%齧齒動物%隱含種%寧夏
DNA조형마%자연역원지%교치동물%은함충%저하
DNA barcoding%nature focus%rodent%implied species%Ningxia
目的：促进宁夏不同地区人兽共患病自然疫源地宿主动物的准确分类和鉴定，提高形态分类的准确性，探讨DNA条形码技术分型的可行性。方法在宁夏不同地区及生境采集啮齿动物标本154份，扩增标本的COI基因部分序列，并进行测序和相关分析。结果捕获的19个形态种的啮齿类动物中，通过COI基因的分析发现20个分子种。形态上鉴定为黑线仓鼠的标本存在2个分子种；贺兰山鼠兔在分子上与褐斑鼠兔比较接近，遗传距离为3．6％；结论 DNA条形码可以用于啮齿动物标本的准确鉴定，纠正形态鉴定的错误；可以发现形态无法区分的隐含种，可以更好的研究啮齿类的分类及进化关系。
目的：促進寧夏不同地區人獸共患病自然疫源地宿主動物的準確分類和鑒定，提高形態分類的準確性，探討DNA條形碼技術分型的可行性。方法在寧夏不同地區及生境採集齧齒動物標本154份，擴增標本的COI基因部分序列，併進行測序和相關分析。結果捕穫的19箇形態種的齧齒類動物中，通過COI基因的分析髮現20箇分子種。形態上鑒定為黑線倉鼠的標本存在2箇分子種；賀蘭山鼠兔在分子上與褐斑鼠兔比較接近，遺傳距離為3．6％；結論 DNA條形碼可以用于齧齒動物標本的準確鑒定，糾正形態鑒定的錯誤；可以髮現形態無法區分的隱含種，可以更好的研究齧齒類的分類及進化關繫。
목적：촉진저하불동지구인수공환병자연역원지숙주동물적준학분류화감정，제고형태분류적준학성，탐토DNA조형마기술분형적가행성。방법재저하불동지구급생경채집교치동물표본154빈，확증표본적COI기인부분서렬，병진행측서화상관분석。결과포획적19개형태충적교치류동물중，통과COI기인적분석발현20개분자충。형태상감정위흑선창서적표본존재2개분자충；하란산서토재분자상여갈반서토비교접근，유전거리위3．6％；결론 DNA조형마가이용우교치동물표본적준학감정，규정형태감정적착오；가이발현형태무법구분적은함충，가이경호적연구교치류적분류급진화관계。
ABSTRACT:To improve the ability of correct classification and identification of rodents in different kinds of foci of zoonosis in Ningxia Hui Autonomous Region in China ,rodents samples from different habitats of Ningxia were collected .Sequences of COI gene were amplified and sequenced from 154 samples .Based on these sequences ,the pairwise genetic distance were calcu‐lated ,and a Neighbor Joining tree were constructed .According to the NJ tree ,20 clusters with high bootstrap support were found from 19 morphological species .The striped dwarf hamsters were divided into two clusters ,which suggested that there were two cryptic species with stripe on the back .The pika from Helan Mountain showed close relationship with Ochotona pal‐lasi ,and the genetic distance was as low as 3 .6% .Results show that DNA barcodes could be used to accurately identify speci‐mens of rodents and correct morphological identification errors .It could discover appearance indistinguishable implied species and could better study the classification and evolution of rodents .
