CAJ | 학술논문

目的：分析我院临床分离非结核分枝杆菌（NTM）菌株，探讨福建省NTM临床分离率和菌种分布情况。方法对我院2009－2012年临床分离6362株分枝杆菌进行结核分枝杆菌复合群（MTBC）与NTM鉴别，对鉴定为NTM菌株应用hsp65‐和rpoB‐PCR‐RFLP进行菌种鉴定。结果6362株临床分离分枝杆菌共鉴别出649株NTM。鉴别出的649株NTM菌株，经hsp65‐和rpoB‐PCR‐RFLP鉴定，菌种分布达24个种，其中菌株数量前3位菌种为：胞内分枝杆菌（M．intracellulare）302株（46．5％），鸟分枝杆菌（M．avium）138株（21．3％），脓肿分枝杆菌（M．abscessus）81株（12．5％）。结论2009－2012年福建省NTM临床分离率占分枝杆菌培养阳性菌株的10．2％，NTM种类多，以鸟‐胞内分枝杆菌复合群（MAC）为福建省NTM的主要流行菌种，占总数的67．8％。
목적：분석아원림상분리비결핵분지간균（NTM）균주，탐토복건성NTM림상분리솔화균충분포정황。방법대아원2009－2012년림상분리6362주분지간균진행결핵분지간균복합군（MTBC）여NTM감별，대감정위NTM균주응용hsp65‐화rpoB‐PCR‐RFLP진행균충감정。결과6362주림상분리분지간균공감별출649주NTM。감별출적649주NTM균주，경hsp65‐화rpoB‐PCR‐RFLP감정，균충분포체24개충，기중균주수량전3위균충위：포내분지간균（M．intracellulare）302주（46．5％），조분지간균（M．avium）138주（21．3％），농종분지간균（M．abscessus）81주（12．5％）。결론2009－2012년복건성NTM림상분리솔점분지간균배양양성균주적10．2％，NTM충류다，이조‐포내분지간균복합군（MAC）위복건성NTM적주요류행균충，점총수적67．8％。
ABSTRACT:To understand the species distribution of nontuberculous mycobacteria (NTM ) in Fujian Province of China , we collected clinical Mycobacterium isolates in the Fuzhou Pulmonary Hospital from 2009 to 2012 .A total of 6 362 clinical My‐cobacteria isolates were identified as 5 713 (89 .8% ) M .tuberculosis complex and 649 (10 .2% ) NTM strains by conventional identification method .Then ,by means of hsp65‐and rpoB‐PCR‐RFLP methods ,649 NTM strains were identified as 24 spe‐cies or complex of NTM ,in which the top three species or complex with the highest occurrence frequency were M .intracellular , M .avium and M .abscessus ,accounting for 48 .5% ,21 .3% and 12 .5% respectively .The prevalence rate of NTM was 10 .2%among Mycobacterium culture‐positive patients .There are lots of NTM species infecting human being ,and the most prevalence NTM species was M .avium complex accounting for 67 .8% in Fujian Province .