CAJ | 학술논문

目的：探讨DNA条形码技术在白蛉物种鉴定中的可行性。方法通过研究白蛉亚科3个属9个物种的线粒体细胞色素C氧化酶亚基I基因序列，以Kimura双参数模型进行种内种间遗传距离分析、使用邻接法构建系统发育树。结果种内平均遗传距离（0．8％）远远小于种间平均遗传距离（11．2％），同种个体聚为高支持度的单一分支。结论基于线粒体COI基因的DNA条形码可以将不同蛉种很好的区分开来，可以作为一种有效的工具在白蛉物种鉴定中应用。
목적：탐토DNA조형마기술재백령물충감정중적가행성。방법통과연구백령아과3개속9개물충적선립체세포색소C양화매아기I기인서렬，이Kimura쌍삼수모형진행충내충간유전거리분석、사용린접법구건계통발육수。결과충내평균유전거리（0．8％）원원소우충간평균유전거리（11．2％），동충개체취위고지지도적단일분지。결론기우선립체COI기인적DNA조형마가이장불동령충흔호적구분개래，가이작위일충유효적공구재백령물충감정중응용。
ABSTRACT:To explore the feasibility of DNA barcoding in the identification of sandflies ,the mitochondrial cytochrome c oxidase subunit I (COI) genes of 9 dominant species in China from three genera in the Phlebotominae were studied .The in‐traspecies and interspecies genetic distances were calculated using the Kimura‐2‐parameter model ,and the phylogenetic trees were reconstructed by neighbor‐joining (NJ) method .Results showed that all of sandflies species were successfully distinguish‐ed by the phylogenetic trees .The average intraspecific genetic distance (0 .8% ) was much less than the average interspecific ge‐netic distance (11 .2% ) .The study based on COI sequences indicates that the sandflies from China could be well distinguished with the commonly used COI barcodes ,which are potentially well utilized in sandflies species identification .